Quint Lab:enzyme lists

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Restriktionsenzyme enzyme isoschizomeres buffer temperature heat inactivated add BSA add SAM add Oligonucleotide star activity AatII (?) 1x NEBuffer 4 37°C 65°C 20' AatII 1x Tango 37°C 65°C 20' Alw26I BsmAI 1x Tango 37°C 65°C 20' Alw26I Fast Digest (?) BsmAI 1x Fast Digest 37°C 65°C 20' AseI VspI NEB3 37°C 65°C 20' BamHI Buffer BamHI 37°C 80°C 20' BamHI Fast Digest 1x Fast Digest 37°C 80°C 5' BauI BssSI 1 x Tango 37°C 65°C 20' BbvI BseXI 1x NEBuffer 2 37°C 65°C 20' BbsI Fast Digest BpiI 1x Fast Digest 37°C 65°C 10' BceAI NEB3 37°C 65°C 20' yes BcuI SpeI 1x Tango 37°C no BfuI BciVI 1x Buffer BfuI 37°C 80°C 20' BpiI BbsI 1x Buffer G 37°C 65°C 20' BglII Fast Digest 1x Fast Digest 37°C no BseGI FokI 1x Tango 55°C 80°C 20' BseMII BspCNI 1x Tango 55°C 80°C 20' yes BseNI BsrI 1x Buffer B 65°C 80°C 20' BseRI 1x NEBuffer 4 37°C 65°C 20' Bsp119I BstBI 1x Tango 37°C 80°C 20' Bsp1407I Fast Digest BsrGI 1x Fast Digest 37°C 80°C 10' BspOI BmtI 1x Buffer O 37°C 80°C 20' BveI BspMI 1x Buffer O 37°C 65°C 20' yes * Cfr10I BsrFI Buffer Cfr10I 37°C no * DpnI Fast Digest BsrFI 1x Fast Digest 37°C 80° 5' * EagI-HF Eco52I 1x NEBuffer 4 37°C 65°C 20' * Eam1104I EarI 1x Tango 37°C 65°C 20' Eco31I Fast Digest 1x Fast Digest 37°C 65°C 5' Eco32I EcoRV 1x Buffer R 37°C 80°C 20' Eco47I AvaII 1x Buffer R 37°C 65°C 20' Eco147I StuI 1x Buffer B 37°C 80°C 20' EcoRI Buffer EcoRI 37°C 65°C 20' EcoRI Fast Digest 1x Fast Digest 37°C 80°C 5' EcoRV Eco32I NEB3 37°C 80°C 20' yes EcoRV Fast Digest 1x Fast Digest 37°C no FspI NsbI 1x NEBuffer 4 37°C 65°C 20' HindIII NEB2 37°C 65°C 20' Hin6I HinP1I 1x Tango 37°C no Hpy188I NEB4 37°C 65°C 20' HpyF3I DdeI 1x Tango 37°C 65°C 20' HpyF10VI MwoI 1x Tango 37°C 80°C 20' KpnI ReACT4 37°C KpnI Fast Digest 1x Fast Digest 37°C 80°C 5' Kpn2I BspEI 1 x Tango 55°C 80°C 20' KspAI HpaI 1x Buffer B 37°C 65°C 20' MboI 1x Buffer R 37°C 65°C 20' MboII 1x Buffer B 37°C 65°C 20' MnlI 1x Buffer G 37°C 65°C 20' MvaI BstNI 1x Buffer R 37°C no NcoI 1x Tango 37°C 65°C 20' NcoI Fast Digest 1x Fast Digest 37°C 65°C 15' PacI (?) NEB1 37°C 65°C 20' yes PaeI SphI 1x Buffer B 37°C 65°C 20' PciI PscI NEB3 37°C 80°C 20' yes PsyI Tth111I 1x Buffer B 37°C 80°C 20' PvuII NEB2 37°C no SacI Buffer SacI 37°C 65°C 20' SacII Cfr42I NEB4 37°C 65°C 20' SalI SuRE/Cut Buffer H 37°C * SapI 1x NEBuffer 4 37°C 65°C 20' SmaI 1x Tango 30°C 65°C 20' ScrFI Bme1390I NEB4 37°C 65°C 20' SpeI Fast Digest (?) BcuI 1x Fast Digest 37°C no TaqI Buffer TaqI 65°C no VspI AseI 1x Buffer O 37°C 65°C 20' XhoI 1x Buffer R 37°C 80°C 20' XhoI Fast Digest 1x Fast Digest 37°C 80°C 5' (?) = Achtung sehr wenig DNA-modifying enzym in stock? supplier, bestnr. jahr T4 DNA ligase y z RevertAid M-MuLV Reverse Transcriptase ja Fermentas, EP0442 2008 x y z x y z