Protein blot (Western) hub

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Revision as of 13:58, 16 April 2009 by Jakob Suckale (talk | contribs) (→General Western blot protocol)
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The protein blot, also know as Western blot or immunoblot, is one of the most tried and true experiments for antibody-dependent determination of protein expression/presence in a sample. This pages directs you both to the general and lab-specific protocols regarding protein blotting.

Contents

  • 1 General protein blot protocol
  • 2 Western blot protocols by individual labs
  • 3 See also
  • 4 External links

General protein blot protocol

  • Protein blot (Western)

Western blot protocols by individual labs

  • Endy:Tris-Tricine Acrylamide Gels
  • Endy:E. coli Western Blot
  • Endy: General Western Blot
  • Odom:Western Blot
  • Sauer:Western blot
  • Ketner: Tissue culture viral lysate Western Blot
  • Tissue preparation for western blot

See also

  • Griffin:Membrane Stripping and Reprobing

External links

  • Western blot on Wikipedia
  • Discussion on Western blots on Protocol Online
  • Quantifying protein blot (Western) band without expensive software - Photoshop, ImageJ
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  • In vitro
  • Protein

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  • This page was last edited on 16 April 2009, at 13:58.
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