User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/25
Procedure Update, MHA Day One | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
July 25, 2013Procedure Update: MHA Silica-CoatingAccording to Aggregation and adhesion of gold nanoparticles in phosphate buffer saline, PBS and prolonged sonication actually cause aggregation in gold nanoparticle solutions: "results showed an optimized sonication time of 10 min to reach equilibrium for plain gold nanoparticles in water." Instead of using PBS to dilute all components, filtered MilliQ water will be used to dilute nanoparticles as well as MHA, as the organic solvent in solution prior to the actual silica-coating may have also been affecting observed the gold nanoparticle aggregation. Filtered MilliQ water will be brought up to room temperature as we've noticed cold solutions (whether water or PBS) have seemed to induce aggregation as well.
Fluorescence Data: 4 and 8 Repeat DNA with ThTFluorescence measurements taken and calibration curve constructed in order to show the increased fluorescence intensity with increases number of G-quadruplexes formed.<br.> <br.> <br.> <br.> <br.> <br.> <br.> |