Kevin T.L. Vogelsang

Plate	plasmid with insert	plasmid no insert
Expected	ZraI	BamHI
Observed	SEBuffer B NEBuffer 1, 2, 4	NEBuffer 2
Temperature	37 C	37 C
Predicted fragments	1 DNA fragment the length of the M13 backbone plus the insert	1 Fragment the lenght of the original M13 genome
		Expected		Observed
Enzyme(s) used	BamHI	ZraI
Buffer used	NEBuffer 2	SEBuffer B; NEBuffer 1,2,4
Temperature	37 C	37 C
Predicted fragments	No linear fragments. Plasmid remains circular.	No linear fragments. Plasmid remains circular.