Eccles: DGG Protocols

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  • siRNA Transfection (Jess Protocol)
  • siRNA Transfection with Lipofectamine 2000 (Aaron)
  • Antisense Transfection (Jess Protocol)
  • cDNA Synthesis (Jess Protocol)
  • qPCR Reaction (Jess Protocol)
  • Culturing NZM Cell Lines (Jess Protocol)
  • DNA Isolation (Jody Protocol)
  • PCR Product Sequencing (Matt Protocol)
  • CO2 for cell culture
  • Random oligo hyb
  • Western Blotting (Lana)
  • Western Blot Stripping (Lana)
  • Protein Lysates from Cells in Culture (Lana)
  • Protein Lysates from Tissue (Lana)
  • Making Primary Cells from Mouse Tissue (Lana)
  • Making Primary Cells from Surgery Tissue (Lana)
  • Culturing CB Cells (Mouse Embryonic Kidney Cells, SV40 large T positive) (Lana)
  • Collagen Coating of Cell Culture Vessels (Lana)
  • Culturing Human ADPKD Primary Kidney Cells (Lana)
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