NanoBio:Protocols
From OpenWetWare
Peptoids
Nano Bioimaging
Nano Synth Bio
Cloning using Biobricks or Biofusion Vectors
- Note: Many of these are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab.
- Strategy for Making Parts
- Designing Parts or Genes
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- Quick & Dirty Digest & Ligation
- Bacterial Transformation
- Plasmid Verification
- Integration into Yeast
- Verifying Genomic Integration
