Griffitts:Protocols

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Recipes

• Common buffers
• Common media
• Stock solutions

DNA

• Qiagen Miniprep
• Low-copy plasmid prep
• PCR primers
• Standard PCR
• Pfx PCR
• Overlap-extension PCR
• Arbitrary PCR
• PCR clean-up (Qiagen)
• Gel electrophoresis
• Restriction digest
• In-gel ligation
• Sequencing prep

RNA

• Trizol purification (bacterial)
• DNAse treatment
• Reverse transcription
• 5'RACE

Protein

• Gel recipes
• Sample buffer
• Bacterial lysis
• 6His purification (6His)
• MBP purification (MBP)

Bacteria

• Media
• Antibiotics
• Tips on streaking/culturing
• Freezing procedure
• Genomic DNA prep
• RNA prep (Trizol)
• ß-Gal/GUS assay
• Triparental Mating
• Transduction
• Competent Cells
• Electroporation

Plants

• Media
• Conetainer Rack Protocol
• Seed scarification and germination
• Tips for growth/seed storage
• DNA prep
• RNA prep
• Seed Storage
• Microscopy