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PKU_080512_G1_1
- Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA
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|
Volume (μL)
|
| DNA |
5
|
| ClaI |
0.2
|
| BamH1 |
0.2
|
| KBuffer |
2
|
| ddH2O |
12.6
|
- PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
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|
Volume (μL)
|
| pGREG505/(TEF-lacI-adhlacS-GFP) |
5
|
| dNTP |
5
|
| KOD Plus |
1
|
| KOD Plus Buffer |
5
|
| MgSO4 |
2
|
| F-Primer |
1
|
| R-Primer |
1
|
| ddH2O |
34
|
- conditions
| Temperature(°C) |
Time Length(0:00:00)
|
| 94 |
05:00
|
| 94 |
00:30 |
30 cycles
|
| 55 |
00:30 |
30 cycles
|
| 72 |
01:30 |
30 cycles
|
| 72 |
07:00
|
| Alarm |
5 times
|
| Hold |
4°C
|
