Talk:20.109(F08): Mod 1 Day 3 Agarose gel electrophoresis
From OpenWetWare
T/R lab
Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised. Nice work!!
And here's the gel with your recovered fragments/backbones, ready for ligation. Thanks to Michelle for running and posting this.
| Lane | Sample | Lane | Sample |
|---|---|---|---|
| 1 | Blue Frag | 8 | Red BKB |
| 2 | Blue BKB | 9 | Orange Frag |
| 3 | Green Frag | 10 | Orange BKB |
| 4 | Green BKB | 11 | Purple Frag |
| 5 | Pink Frag | 12 | Purple BKB |
| 6 | Pink BKB | 13 | Yellow Frag |
| 7 | Red Frag | 14 | Yellow BKB |
W/F lab
Drum roll.....Here's the W/F gel of the recovered products.
| Lane | Sample | Lane | Sample |
|---|---|---|---|
| 1 | Blue Frag | 8 | Yellow BKB |
| 2 | Blue BKB | 9 | Purple Frag |
| 3 | Green Frag | 10 | Purple BKB |
| 4 | Green BKB | 11 | Ladder |
| 5 | Pink Frag | 12 | |
| 6 | Pink BKB | 13 | |
| 7 | Yellow Frag | 14 |
