Materials

Media

Defined Media

1. M9 salts
2. M9 media
3. M9 supplemented media
4. SD

Standard Media

1. LB
2. T Broth
3. YPD

Enzymes

Phosphatases

1. Antarctic Phosphatase
2. Alkaline Phosphatase

RNA Polymerases

1. T7 RNA Polymerase
2. T4 RNA Polymerase

DNA Polymerases

1. Vent
2. Taq

Reporters

Dyes and stains

Useful links

http://www.probes.com/handbook/sections/0801.html
http://www.probes.com/handbook/sections/1502.html

Individual dyes

1. Acridine Orange
2. Carboxyfluorescein Diacetate
3. DAPI
4. Fluorescein diacetate
5. Hoechst 33342
6. Propidium iodide
7. SYBR Green I
8. SYBR Green II
9. SYTO

Notes

There don't appear to be any dyes that selectively stain live cells. When people want to detect live cells, I think that what they do is the following: "The principle of this approach is to use simultaneously a permeant (SYBR Green [more recently SYTO green]; Molecular Probes) and an impermeant (propidium iodide) probe and to take advantage of the energy transfer which occurs between them when both probes are staining nucleic acids. A full quenching of the permeant probe fluorescence by the impermeant probe will point to cells with a compromised membrane, a partial quenching will indicate cells with a slightly damaged membrane, and a lack of quenching will characterize intact membrane cells identified as viable." From Applied and Environmental Microbiology, October 2001, p. 4662-4670, Vol. 67, No. 10.