Talk:20.109(S09): Luciferase assays and RNA prep (Day6)
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T/R Luciferase Reactions
Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (Renilla/firefly).
Note: feel free to put ratios from either background-subtracted or raw values; they are likely to be quite similar. Hoewever, if you see this note, please mark which kind of data your ratios come from.
Team Colour | none | pLuc only | validated | scrambled | experimental | raw or subtracted? | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | ||
Pink | 4.5 | 4.0 | 20.7 | 19.1 | 2.73 | 1.92 | 18.9 | 22.9 | 14.7 | 17.0 | |
Green | .94 | .97 | 36.4 | 26.3 | 2.7 | 2.4 | 27.6 | 29.1 | 2.8 | 2.6 | Raw |
Orange | .076 | 22.3 | 21.2 | 29.0 | 2.25 | 1.74 | 22.0 | 22.8 | 4.8 | 4.1 | |
Yellow | 1.33 | .17 | 20.8 | 29.4 | 1.42 | 2.24 | 25.74 | 26.08 | 1.17 | 1.53 | |
Grey | 1.74 | 1.10 | 38.64 | 34.59 | 2.55 | 2.90 | 37.75 | 35.83 | 9.04 | 8.80 | Raw |
Red | 2.02 | 3.00 | 21.36 | 16.40 | 1.31 | 1.51 | 20.78 | 23.90 | 1.53 | 1.61 | Raw |
Blue | 1.57 | 3.64 | 14.78 | 18.67 | 1.75 | .51 | 17.40 | 15.16 | 8.25 | 6.87 | |
W/F Luciferase Reactions
Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (Renilla/firefly).
Note: feel free to put ratios from either background-subtracted or raw values; they are likely to be quite similar. Hoewever, if you see this note, please mark which kind of data your ratios come from.
Team Colour | none | pLuc only | validated | scrambled | experimental | raw or subtracted? | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | ||
Pink | 1.83 | 1.39 | 22.59 | 20.86 | 2.87 | 2.91 | 21.06 | 21.61 | 18.10 | 18.08 | |
Orange | 1.11 | 0.89 | 20.56 | 11.30 | 1.70 | 2.30 | 21.71 | 16.40 | 6.42 | 5.13 | |
Red | 2.133 | 2.6 | 5.924 | 14.4 | 0.385 | 1.636 | 20.96 | 19.89 | 1.96 | 1.72 | |
Blue | 1.019 | 0.761658 | 16.17009 | 19.39064 | 2.758433 | 8.679711 | 15.43884 | 10.86473 | 14.8144 | 12.87329 | |
Purple | 1.1016 | 1.820 | 32.05 | 30.98 | 1.369 | 2.073 | 35.384 | 33.298 | 1.189 | 1.751 | raw |
Green | 0.878 | 1.028 | 31.548 | 23.173 | 1.562 | 3.331 | 24.934 | 18.317 | 4.434 | 9.589 | Raw |
Yellow | 1.071 | 0.942 | 29.831 | 29.021 | 3.040 | 3.575 | 30.356 | 31.690 | 1.989 | 2.203 | RAW |