PrbbBB:Protocols
From OpenWetWare
<html>
<colgroup width=126 span=6>
</colgroup>
| <a href="PrbbBB">Home | <a href="http://openwetware.org/wiki/PrbbBB:Members">Members | <a href="http://openwetware.org/wiki/PrbbBB:Protocols">Protocols | <a href="http://openwetware.org/wiki/PrbbBB:Internal">Internal | <a href="http://openwetware.org/wiki/PrbbBB:Contact">Contact |
</html>
Biobrick assembly & related
We have streamlined the standard Biobrick assembly protocols. Our "Barcelona set" of protocols is optimized for performing many assemblies in parallel.
- prepare vector backbone for assembly (Freiburg Fusion format)
- Assembly protocol for BioBricks on 96 well plate (Freiburg Fusion format)
- Colony PCR screening of many assemblies in parallel (on 96 well plate)
- Ligation-free Biobrick Construction by PCR/recombination (Freiburg Fusion format)
- DNA measurement on plates
