Smolke:Protocols/DNA purification

Home        Contact        Internal        Protocols        Lab Members        Publications        Research       

Situations to use a column-based purification kit Cleaning up PCR products after each step in assembly PCR. --YC Cleaning up reverse transcription products during SELEX. --YC When I'm in an absolute time crunch, which is not often. --YC Situations to use phenol-chloroform extraction & ethanol precipitation Cleaning up and concentrating PCR-amplified inserts for cloning. --YC Cleaning up after digestion. --YC You could also just do ethanol precipitation without phenol-chloroform extraction if you are simply trying to reduce the volume of a sample (for subsequent gel extraction, for example). I believe some people also just put it in the speed vac for this purpose. --YC Large quantities of DNA (>10ug). -JKM