Quint Lab:enzyme lists

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Restriktionsenzyme

enzyme	isoschizomeres	buffer	temperature	add BSA	add SAM	add Oligonucleotide	star activity
AatII		1x NEBuffer 4	37°C
Alw26I	BsmAI	1x Tango	37°C
Alw26I Fast Digest	BsmAI	1x Fast Digest	37°C
AseI	VspI	NEB3	37°C
BamHI		Buffer BamHI	37°C
BamHI Fast Digest		1x Fast Digest	37°C
BcuI	SpeI	1x Tango	37°C
BseGI	FokI	1x Tango	55°C
BseMII	BspCNI	1x Tango	55°C		yes
BseNI	BsrI	1x Buffer B	65°C
Bsp119I	BstBI	1x Buffer B	37°C
BspOI	BmtI	1x Buffer O	37°C
Cfr10I	BsrFI	Buffer Cfr10I	37°C				*
EagI-HF	Eco52I	1x NEBuffer 4	37°C
EcoRI		Buffer EcoRI	37°C
EcoRI Fast Digest		1x Fast Digest	37°C
EcoRV	Eco32I	NEB3	37°C	yes
FspI	NsbI	1x NEBuffer 4	37°C
HindIII		1x Buffer R	37°C
HindIII		NEB2	37°C
Hin6I	HinP1I	1x Tango	37°C
Hpy188I		NEB4	37°C
HpyF3I	DdeI	1x Tango	37°C
HpyF10VI	MwoI	1x Tango	37°C
KpnI		ReACT4	37°C
KpnI Fast Digest		1x Fast Digest	37°C
KspAI	HpaI	1x Buffer B	37°C
MboI		1x Buffer R	37°C
MnlI		1x Buffer G	37°C
NcoI		1x Tango	37°C	no	no
NcoI Fast Digest		1x Fast Digest	37°C	no	no
PacI		NEB1	37°C	no	yes
PsyI	Tth111I	1x Buffer B	37°C	no	no
PvuII		NEB2	37°C	no	no
SacI		Buffer SacI	37°C	no	no
SacII	Cfr42I	NEB4	37°C	no	no
SalI		SuRE/Cut Buffer H	37°C	no	no	*
SapI		1x NEBuffer 4	37°C	no	no
SmaI		1x Tango	30°C	no	no
SpeI Fast Digest	BcuI	1x Fast Digest	37°C	no	no
TaqI		Buffer TaqI	65°C	no	no
VspI	AseI	1x Buffer O	37°C	no	no
XhoI Fast Digest		1x Fast Digest	37°C	no	no

enzym	in stock?	supplier, bestnr.	jahr
T4 DNA ligase	y	z
RevertAid M-MuLV Reverse Transcriptase	ja	Fermentas, EP0442	2008
x	y	z
x	y	z