IGEM:MIT/2006/Notebook/2006-6-8
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PRIMER INFORMATION FOR ORDERING
SAMT from A. Majus
- Forward Strand
- Sequence to order: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG ACA AAA CAA ACA CAA AAG C -3'
- Length of matching: 21 bp
- GC content: 28.6%
- Melt Temp. 48.0 C
- Reverse Strand
- Sequence to order: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TA GTT TCT TTT TGC TTG TCA CTC -3'
- Length of matching: 21 bp
- GC content: 38.1.0%
- Melt Temp. 50.4 C
BAMT from A. majus
- Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAA GTG ATG AAG AAA C
- Length: 47
- GC Content: 31.6%
- Melting Temp: 45.2
- Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TA TTA TTA TCT CCT ACT TAG AGA AAC
- Length: 53
- GC Content: 38.9%
- Melting Temp: 44.3
BSMT from P. x hybrida
Designed Forward Strand (1 and 2):
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG GAA GTT GTT GAA GTT C -3'
- LENGTH: 49
- GC CONTENT: 46.9 %
- MELT TEMP: 67.6 ºC
Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAC -3'
- LENGTH: 51
- GC CONTENT: 39.2 %
- MELT TEMP: 65.0 ºC
Designed Reverse Strand (phBSMT2): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAA C -3'
- LENGTH: 52
- GC CONTENT: 38.5 %
- MELT TEMP: 65.0 ºC
ATF1 from S. Cerevisiae
Designed forward primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAT GAA ATC GAT GAG -3'
- Length: 46 bp
- GC content: 45.7%
- Melt temp: 66.4 deg. C
Designed reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA GGG CCT AAA AGG AGA G -3'
- Length: 52 bp
- GC content: 48.1%
- Melt temp: 67.9 deg. C