SBB10Ntbk-JoseGutierrez

My Project

Construction Files

SBB30: 5'UTR_rep009

Biobricking of O99 rep Gene
PCR JG001-F and JG002-R on pEC52      (1235 bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144             (EcoRI/BamHI, 910+2472, L)
Product is pBca9523-sbb30    {5'UTR_repO99!}
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JG001-F   Cloning of O99 rep Gene  
ccataGAATTCatgAGATCTaaaaacgattctgacgcattttttatg
JG002-R   Cloning of  O99 rep Gene   
CTGATGGATCCCTACTCTACAAGACCTCGTTTTttc

SBB38: PBad Promoter

Eco/Bam transfer pBjh1601CK-ig114
Sub into pBjk2741-Bca1144                      (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-ig114   {AraC-Pbad}

SBB37: PCon Promoter

Eco/Bam transfer pBca1100-Bca1152
Sub into pBjk2741-Bca1144                      (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-Bca1152   {Pcon-J23100}