Berk2010-Group
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Amy N. Kristofferson 17:15, 7 June 2010 (EDT)
First step of Manual Assembly
Lefty MasterMix
- 4uL miniprep DNA
- 4uL water
- 1uL of NEB2
- 0.5uL XhoI
- 0.5uL BamH1
Righty MasterMix
- 4uL miniprep DNA
- 4uL water
- 1uL of NEB2
- 0.5uL XhoI
- 0.5uL BglII
Christoph Neyer 16:54, 7 June 2010 (EDT)
Some of Tim's spreadsheet data (which clones have been chosen for 2ab assembly):
Chosen clone (June2) Chosen clone (June3)
4 AC 9 B10sbb20 <FokI-! 1 1
4 AC 10 B10sbb43 {rbs2_lamB>} 2 1
4 AC 16 B10sbb15 phiC31 attB 2 1 seq
4 AC 17 B10sbb14 rbs_phiC31> 3 1
5 4 AC 18 B10sbb13 <phiC31> 3 1
7 AK 2 B10sbb09 Tn5 3'TR 4 1 seq
7 AK 5 B10sbb06 piggieBac3'TR 4 1
7 AK 9 B10sbb20 <FokI-! 5 1
7 AK 10 B10sbb43 {rbs2_lamB>} 5
7 AK 11 Bjh1906 b1006 6 1 seq
7 AK 13 B10sbb18 <zf-> 7 1
7 AK 14 B10sbb17 <zf+> 8 1
7 AK 19 B10sbb12 sleeping_beauty_3'TR 8 1
7 AK 23 B10sbb42 {rbs_pelB>} 9 d
10 7 AK 24 Bjh2294 Self_Lysis_Device 9 c
9 CA 3 Bjh1858 <NLS! 10 1 seq
9 CA 8 ig114 {AraC-Pbad} 11 1
9 CA 11 Bjh1906 b1006 11 a seq
9 CA 13 B10sbb18 <zf-> 11 1
9 CA 20 B10sbb44 Promoter_rbs_GenR 12 2
6 9 CA 23 B10sbb42 {rbs_pelB>} 13 a seq
5 CK 3 Bjh1858 <NLS! 13 1 seq
5 CK 8 ig114 {AraC-Pbad} 13 1
5 CK 15 B10sbb16 phiC31 attP 14 1 seq
5 CK 20 B10sbb44 Promoter_rbs_GenR 15 2
5 5 CK 23 B10sbb42 {rbs_pelB>} 16 1 seq
6 KA 2 B10sbb09 Tn5 3'TR 17 2 seq
6 KA 4 B10sbb07 <tn5> 17 2
6 KA 5 B10sbb06 piggieBac3'TR 18 2
6 KA 7 B10sbb04 <piggyBac! 18 2
6 KA 11 Bjh1906 b1006 19 1 seq
6 KA 12 B10sbb19 <FokI+! 19 2 seq
6 KA 13 B10sbb18 <zf-> 20 2
6 KA 19 B10sbb12 sleeping_beauty_3'TR 20 2
6 KA 22 B10sbb10 <SB100x! 21 1
6 KA 23 B10sbb42 {rbs_pelB>} 22 2
11 6 KA 24 Bjh2294 Self_Lysis_Device 23 a
8 KC 1 B10sbb08 Tn5 5'TR 23 1 seq
8 KC 4 B10sbb07 <tn5> 23 2
8 KC 6 B10sbb05 piggieBac_5'TR 23 1
8 KC 17 B10sbb14 rbs_phiC31> 24 2
8 KC 18 B10sbb13 <phiC31> 24 1
6 8 KC 21 B10sbb11 sleeping_beauty_5'TR 24 1
