IGEM:MIT/2006/Notebook/2006-6-20

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Revision as of 15:24, 20 June 2006 by Boyuanzhu (talk | contribs) (→To do)
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To do

  1. Set up and run gradient PCR for BAMT and SAMT
  2. run test gels on exhaustive BAMT and SAMT pcr products - possibly reconsider primers
  3. Reconsider ATF1 primers definitely
  4. Pour new minimal plates
  5. Get in touch with GC lab
          • 3 pm: Stephen has meeting --- light sensor part!

WINTERGREEN!!!!!!!

  1. The liquid cultures from yesterday SMELLED LIKE WINTERGREEN!! SO EXCITING!!!! YAY!
  2. The SAMT+SA and the BSMT+SA worked, in both LB and minimal media
    • Now it's time to optimize SA concentration :)
  3. Plated liquid cultures onto M9 with 50, 100, 200 μL SA
    • 2 sets of 4 (BSMT M9, BSMT LB, SAMT M9, SAMT LB) with one set of 3 missing BSMT M9

Repeat Smell Experiment

Control BAMT/BA SAMT/SA BSMT/BA BSMT/SA
10mL LB + 20μL BL21 cells + 8.2μL BA [in a tube] 10mL LB Kan + 20μL BAMT cells + 8.2μL BA [in a tube] 10mL LB Kan + 20μL SAMT cells + 6.2μL SA [in a tube] 10mL LB Kan + 20μL BSMT cells + 8.2μL BA [in a tube] 10mL LB Kan + 20μL BSMT cells + 6.2μL SA [in a tube]
10mL minimal media + 20μL BL21 cells + 8.2μL BA [in a tube] 10mL minimal media Kan + 20μL BAMT cells + 8.2μL BA [in a tube] 10mL minimal media Kan + 20μL SAMT cells + 6.2μL SA [in a tube] 10mL minimal media Kan + 20μL BSMT cells + 8.2μL BA [in a tube] 10mL minimal media Kan + 20μL BSMT cells + 6.2μL SA [in a tube]
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