IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-7-16

Container v3.2

c3.2 Folding Experiment

Reagents (each expt in respective 0.2 mL PCR tube)

Experiment	Scaffold	Oligos	Folding buffer	dH2O	total volume
B	9 μL p7308	16 μL 3.2.B	4 μL 10x	11 μL	40 μL
C	9 μL p7308	16 μL 3.2.C	4 μL 10x	11 μL	40 μL
6hb	4.5 μL p7308	2 μL 6hb.v5 (0.99 μM)	2 μL 10x	11.5 μL	20 μL

Annealing protocol

• start at 80[[:Category:{{{1}}}|{{{1}}}]]
• 60 cycles: wait 2 minutes, decrease 1[[:Category:{{{1}}}|{{{1}}}]]
• hold at 4[[:Category:{{{1}}}|{{{1}}}]]