Biomod/2011/TUM/TNT/LabbookA/list TEM pictures

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       TUM NanU - Home

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http://openwetware.org/index.php?title=Biomod/2011/TUM/TNT/LabbookA/list_TEM_pictures&action=edit

summary of all prepared and observed grids

All dilutions were made with FOB20. For the calculation of the intercalator concentration read more in the corresponding labbook entry.


BIOMOD1 grid box

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<colgroup> <col width=5%> <col width=5%> <col width=42,5%> <col width=5%> <col width=42,5%> </colgroup>
grid number   prepared   annotations   observed   annotation
A12345
A22345
A32345
A42345
A52345
A62345
A72345
A82345
A92345
A102345
B12345
B22345
B32345
B42345
B52345
B62345
B72345
B82345
B92345
B102345
C12345
C22345
C32345
C42345
C52345
C62345
C72345
C82345
C92345
C102345
D12345
D22345
D32345
D42345
D52345
D62345
D72345
D82345
D92345
D102345
E12345
E22345
E32345
E42345
E52345
E62345
E72345
E82345
E92345
E102345

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BIOMOD2 grid box

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<colgroup> <col width=5%> <col width=5%> <col width=42,5%> <col width=5%> <col width=42,5%> </colgroup>
grid number   prepared   annotations   observed   annotation
A1oct. 5thBM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)oct. 7thgood stain, slightly to high structure concentration, 60 images
A2oct. 5thBM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)oct. 7thgood stain, slightly to high structure concentration, 60 images
A3oct. 5thBM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)oct. 7thgood stain, slightly to high structure concentration, 60 images
A4oct. 5thBM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)oct. 7thgood stain, slightly to high structure concentration, 60 images
A5oct. 5thBM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)oct. 7thgood stain, slightly to high structure concentration, 60 images
A6oct. 20thBM2 with 432 nM DAPI (corresponding to one molecule every 7bp), 1:25oct. 28stno origamis on the grid
A7oct. 20thBM2 with 144 nM DAPI (corresponding to one molecule every 21bp), 1:25oct. 27thno origamis on the grid
A8oct. 20thBM2 with 144 nM DAPI (corresponding to one molecule every 21bp), 1:25 oct. 28thno origamis on the grid
A9oct. 20thBM2 without any DNA binders, 1:25oct. 21thstain was good
A10oct. 20thBM2 without any DNA binders, 1:25oct. 21thstain was good
B1oct. 5thBM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)oct. 12thgrid was fine
B2oct. 10thBM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)oct. 12thtoo high dilution
B3oct. 10thBM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)45
B4oct. 10thBM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)45
B5oct. 10thBM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)45
B6oct. 20thBM2 without any DNA binders, 1:25oct. 21thstain was good
B7oct. 20thBM2 without any DNA binders, 1:25oct. 21thstain was good
B8oct. 20thBM24 15_65 1:25 without any DNA bindersoct. 20thbad stain and to high dilution
B9oct. 20thBM24 2DH3ML 1:25 without any DNA binders oct. 20thbad stain, no structures on the grid
B10oct. 21thBM24 15_65 1:20 without any DNA bindersoct. 21thstain and dilution was good
C1oct. 10thBM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)45
C2oct. 10thBM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)45
C3oct. 10thBM2 1:40 without any DNA binders45
C4oct. 10thBM2 1:40 without any DNA binders45
C5oct. 10thBM2 1:40 without any DNA binders45
C6oct. 21thBM24 15_65 dilution 1:20 without any DNA bindersoct. 21thstain was bad
C7oct. 21thBM24 2DH3ML dilution 1:20 without any DNA binders oct. 21thstain was good but dilution slightly to high
C8oct. 21thBM24 2DH3ML 1:20 without any DNA bindersoct. 21thstain was good but dilution slightly to high
C9oct. 27thBM2 1:25 without any DNA binders45
C10oct. 27thBM2 1:25 without any DNA bindersoct. 27thfine grid, 60 images
D1oct. 12thBM2 1:25 with 2.4 µM ethidium bromide (corresponding to one molecule every 7bp)oct. 12thgood stain and dilution
D2oct. 12thBM2 1:25 with 0.74 µM ethidium bromide (corresponding to one molecule every 21bp)oct. 12thgood stain and dilution
D3oct. 12thBM2 1:25without any DNA binders oct. 12thgood stain and dilution
D4oct. 14thBM2 1:25 with 432 µM DAPI (corresponding to one molecule every 7bp)oct. 20thstain and dilution was good, cluster
D5oct. 14thBM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)oct. 20thstain and dilution was good, cluster
D6oct. 27thBM24 (15_65), 1:20 diluted with FOB2045
D7oct. 27thlost the grid before sample preparation45
D8oct. 27thBM24 (15_65) 1:10 without any DNA bindersoct. 27thlow sample concentration, 24 images
D9oct. 27thBM24 (15_65) 1:10 without any DNA binders45
D10oct. 28stBM24 (15_65) 1:5 without any DNA bindersoct. 28st better sample concentration than 2D6 and 2D8, 40 images
E1oct. 20thBM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)oct. 26thgood dilution, bad staining
E2oct. 20thBM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)oct. 26thstrong background
E3oct. 20thBM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)oct. 26thfine grid
E4oct. 20thBM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)oct. 26thfine grid
E5oct. 20thBM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)oct. 26thweak stain
E6oct. 28stBM24 (15_65), 1:5oct. 28stfine grid, 100 images
E7oct. 31thBM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)oct. 31th5
E8oct. 31thBM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)oct. 31th5
E92345
E102345

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