Silver: Protocols

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Genome Organization

• S. cerevisiae Protocols
  • ChIP-chip
  • FISH/IF
  • Immunofluorescence
  • LiOAc Transformation
  • Propidium Iodide FACS

RNA "Stuff"

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Synthetic Biology

• BioBrick Strategy
• PCR
• Oligonucleotide Inserts
• Restriction Digest
• Bacterial Colony PCR
• Ligation
• Bacterial Transformation
• Plasmid Verification

Cell-Based Screens

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Methylation

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Other / More General

Yeast Protocols

• Lysate for Western
• Colony PCR
• Time-Lapse Microscopy