BME103:T930 Group 3 l2
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
PCR Protocol 1.) Get 3 replicate DNA samples each from two patients and one positive control and negative control sample for a total of 8 samples. Mix each of your samples with Taq DNA polymerase, MgCl2, dNTP's, forward primer and reverse primer. Each sample should be about 50 micro liters. 2.)Label 8 empty PCR tube with unique labels that correspond to their respective DNA samples. 3.)Using one pipette per sample, to avoid contamination, transfer the PCR reaction mix to PCR tubes. 4.)Then place the samples into the PCR machine 5.)Set the PCR program to three stages. Stage one: 1 cycle, 95 degree Celsius for 3 minutes. Stage 2: 35 cycles, 95 degrees Celsius for 30 seconds, 57 degrees Celsius for 30 seconds, 72 degrees Celsius. Stage three: 72 degrees Celsius for 3 minutes and then hold at 4 degree Celsius
DNA Measurement Protocol Research and DevelopmentBackground on Disease Markers 1.) The amyloid Beta precursor protein for Alzheimer's - 2.) Ubiquitin-like Modifier-activating enzyme for Spinal muscular atrophy - Primer Design 1.) Forward primer: CTTC[G]ACCT
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