SYBR Green Dye [A short summary describing SYBR green dye]
Single-Drop Fluorimeter [A PHOTO and description of the single-drop fluorimeter device]
How the Fluorescence Technique Works [In your own words, a summary of the information from page 9 of the worksheet]
Procedure
Smart Phone Camera Settings
[The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
Flash:
ISO setting:
White Balance:
Exposure:
Saturation:
Contrast:
[If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
Flash:
ISO setting:
White Balance:
Exposure:
Saturation:
Contrast:
Calibration
[Place a PHOTO that shows your camera phone set up in front of the flourimeter here]
Distance between the smart phone cradle and drop =
[See worksheet page 5]
Solutions Used for Calibration[See worksheet page 5]
Calf Thymus DNA solution concentration (microg/mL)
Volume of the 2X DNA solution (uL)
Volume of the SYBR GREEN I Dye Solution (uL)
Final DNA concentration in PicoGreen Assay (ng/mL)
0
80
80
blank
.25
80
80
.125
.5
80
80
.25
1
80
80
.5
2
80
80
1
5
80
80
2.5
Placing Samples onto the Fluorimeter
[Step one, in your OWN words]
[Step two, in your own words]
[Step three, in your own words]
[etc.]
Data Analysis
Representative Images of Samples
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples[See worksheet page 5]
row 1 cell 1
row 1 cell 2
row 1 cell 3
row 1 cell 4
row 1 cell 5
row 2 cell 1
row 2 cell 2
row 2 cell 3
row 2 cell 4
row 2 cell 5
row 3 cell 1
row 3 cell 2
row 3 cell 3
row 3 cell 4
row 2 cell 5
[Add more rows as needed]
Fitting a Straight Line [Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]