Smolke:Protocols/DO Media

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Recipe Amino Acid Stock Concentration (mg/L) Volume Added (mL/L) Final Concentration (mg/L) Notes Adenine sulfate 200 100 20 Stored at RT Uracil 200 100 20 L-Tryptophan 1000 20 20 L-Histidine hydrochloride 1000 20 20 L-Arginine hydrochloride 1000 20 20 L-Methionine 1000 20 20 L-Tyrosine 200 150 30 L-Leucine 1000 60 60 L-Isoleucine 1000 30 30 L-Lysine hydrochloride 1000 30 30 L-Phenylalanine 1000 50 50 Stored at RT L-Glutamic acid 1000 100 100 Stored at RT L-Aspartic acid 1000 100 100 Stored at RT L-Valine 3000 50 150 L-Threonine 4000 50 200 L-Serine 8000 50 400 Nanopure Water Remainder Notes Leave out whichever amino acid(s) you're dropping out, and replace the volume with water. Stocks which are stored at RT are in the cabinet with autoclaved media. Contact Win