Altman:Protocols/Single Molecule Assays/gliding filament

Department of Physics, Willamette University

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Making Nitrocellulose Coverslips

// Typical flow-through volumes are 10-15 μL //

1. Make a flow cell with a nitrocellulose-coated coverslip 2. Flow in α-GFP/10 3. Incubate 2 minutes 4. Flow in ABSA 5. Incubate 2 minutes 6. Flow in motor dilution 7. Incubate 2 minutes 8. Flow in ABSA 9. Flow in Actin dilution 10. Incubate 2 minutes 11. Flow in GO-Juice

Recipes

	50 uL Buffer #1
50x GOC stock	2 μL
100x CPK stock	1 μL
100x ATP stock	1 μL
AB	46 μL

	50 μL Buffer #2
50x Glu/B stock	2 μL
100x PCr stock	1 μL
AB	47 μL

	20 μL GO Juice
Buffer #1	10 μL
Buffer #2	10 μL