User:Emzodls
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20.109 Spring 2007
Last Name
de los Santos
First Name
Emmanuel Lorenzo
Nickname
Emzo
Course/Minor
20
Year of Graduation
2009
emzodls AT mit DOT edu
Subjects this Term
20.109
7.05
6.001
6.041
21F.101
Module 1 : M13 Genome Re-engineering
M13 Redesign Ideas
| Gene | function | design ideas |
|---|---|---|
| I | assembly | modify genes I, IV and XI to allow other useful proteins to attach to the complex (more flexibility in phage modification |
| II | replication of DNA + strand | add some kind of control to deactivate protein (e.g. sensitivity to heat or a chemical stimuli), this allows better control of the phages |
| III | phage tail protein | add myc epitope, or another marker that can be used for experimentation |
| IV | assembly | (see modification for I) |
| V | binds ssDNA | add tag to track changes, change structure to allow more DNA to fit in the phage |
| VI | phage tail protein | bind it tighter to phage body, modify protein to allow more modifications to p3 |
| VII | phage head protein | (see p6), make protein 9 more flexible to modifications |
| VIII | phage coat protein | add myc or another tag (x-ray sensitive, UV sensitive, flourescent) |
| IX | phage head protein | tag, allow p9 to attach to different kinds of proteins including p3 (makes virus chains) |
| X | DNA replication | add another way to control phage propagation |
| XI | assembly | (see modification for I) |
