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Current revision (13:12, 31 August 2013) (view source)
 
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[["Sponsors" | <font face="trebuchet ms" style="color:#FFFFFF" size="4">'''Sponsors''' </font>]]  
[["Sponsors" | <font face="trebuchet ms" style="color:#FFFFFF" size="4">'''Sponsors''' </font>]]  
</div>
</div>
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[[Image:大見出し.png|30 px]]<font size="5">Experiment 1[[Image:大見出し.png|30 px]] Preparation of Ring-shaped microtubule assemblies</font>
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To obtain ring-shaped microtubules, we perform dynamic self-assembly on kinesin-coated glass surface. <br>
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Under an appropriate concentration of each solutions (200 nM kinesin, 5 microM microtubule (60 % biotinilated),<br>
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300 nM streptavidin, 5 mM ATP ), rotating ring-shaped microtubules were successfully formed (Fig. 1-1).<br>
 +
 +
[[Image:Rimgs.jpg| 500 px]]<br>
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<font size="4">Fig. 1-1 Fluorescent image of ring-shaped microtubule formed on kinesin-coated surface.</font><br>
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<br>
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To understand the properties of the ring-shaped microtubules, we measure their diameter and velocity of rotation. <br>
 +
Histograms based on each results were prepared and showed below (Fig. 1-2 ,1-3).<br>
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<font size="4">
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[[Image:Diameter.png| 400px]]
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<br>Fig. 1-2 Histogram of the diameter of ring-shaped microtubules. <br>
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<br><br>
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[[Image:Velocity.png| 500px]]
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<br>Fig. 1-3 Histogram of the velocity of rotation.<br>
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</font>
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The size and the velocity of ring-shaped microtubule assemblies were stable and good enough to continue our experiments. <br>
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----
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[[Image:大見出し.png|30 px]]<font size="5">Experiment 2 [[Image:大見出し.png|30 px]]Preparation of Marimo-gel</font>
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<br>This experiment was performed by following the method on “materials” page.<br>
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<font size="4">
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[[Image:marimogel.png|400px]]<br>
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Fig. 2-1 Schematic illustration of preparing Marimo-gel.<br><br>
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[[Image:marimo-gel.png|400px]]<br>
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Fig. 2-2 Micrograph of Marimo-gel prepared in this experiment.<br><br>
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[[image:Histogram_of_diameter_of_marimo.png|400px]]<br>
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Fig. 2-3 Histogram of diameter of Marimo-gel.<br><br>
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</font>
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<p>
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Marimo-Gel obtained in this experiment is very much larger than we expected. The diameter should be around several tens <br>
 +
micrometers size to introduce into the flowcell where the ring-shaped microtubules were formed. Also the shape of Marime-gel <br>
 +
was not a good globule.<br>
 +
To prepare smaller and spherical Marimo-gel, we are planning to try another method. By using an appropriate surfactant, <br>
 +
o/w emulsion system seems to us is suitable to prepare micro-Marimo-Gel.<br>
 +
</p>
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[[Image:大見出し.png|30 px]]<font size="5">Experiments3 [[Image:大見出し.png|30 px]]Preparation of micro-sized gear</font><br>
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To obtain micrometer-sized gear, we perform lift-off methods for MEMS devices.<br>
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[[Image:20130829_145254.jpg| 500px]]
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[[Image:20130829 151351.jpg|500 px]]<br>
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<font size="4">Fig. 3-1 Micro-sized gear prepared by lift off method.<br><br>
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</font><br>
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We successfully prepare the micrometer-sized gear.

Current revision

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Experiment 1 Preparation of Ring-shaped microtubule assemblies

To obtain ring-shaped microtubules, we perform dynamic self-assembly on kinesin-coated glass surface.
Under an appropriate concentration of each solutions (200 nM kinesin, 5 microM microtubule (60 % biotinilated),
300 nM streptavidin, 5 mM ATP ), rotating ring-shaped microtubules were successfully formed (Fig. 1-1).


Fig. 1-1 Fluorescent image of ring-shaped microtubule formed on kinesin-coated surface.

To understand the properties of the ring-shaped microtubules, we measure their diameter and velocity of rotation.
Histograms based on each results were prepared and showed below (Fig. 1-2 ,1-3).


Fig. 1-2 Histogram of the diameter of ring-shaped microtubules.



Fig. 1-3 Histogram of the velocity of rotation.

The size and the velocity of ring-shaped microtubule assemblies were stable and good enough to continue our experiments.


Experiment 2 Preparation of Marimo-gel
This experiment was performed by following the method on “materials” page.


Fig. 2-1 Schematic illustration of preparing Marimo-gel.


Fig. 2-2 Micrograph of Marimo-gel prepared in this experiment.


Fig. 2-3 Histogram of diameter of Marimo-gel.

Marimo-Gel obtained in this experiment is very much larger than we expected. The diameter should be around several tens
micrometers size to introduce into the flowcell where the ring-shaped microtubules were formed. Also the shape of Marime-gel
was not a good globule.
To prepare smaller and spherical Marimo-gel, we are planning to try another method. By using an appropriate surfactant,
o/w emulsion system seems to us is suitable to prepare micro-Marimo-Gel.

Experiments3 Preparation of micro-sized gear
To obtain micrometer-sized gear, we perform lift-off methods for MEMS devices.


Fig. 3-1 Micro-sized gear prepared by lift off method.


We successfully prepare the micrometer-sized gear.

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