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<ul>
<ul>
<li>To get the target to move between Selectors, we waited one hour before the next step today. We did electrophoresis at 50V in a fridge (at 4 degrees).</li>
<li>To get the target to move between Selectors, we waited one hour before the next step today. We did electrophoresis at 50V in a fridge (at 4 ℃).</li>
<li>We stain the 10% gel with sybr gold.</li>
<li>We stain the 10% gel with sybr gold.</li>
<li>The bands in the 20% gel were warped, and we couldn't get any sufficient result.</li>
<li>The bands in the 20% gel were warped, and we couldn't get any sufficient result.</li>

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  • In illustration, target means Target DNA<br>
  • In illustration, numbers below the gel pictures signify the interval between the time when the experiment starts and the time when the sample (shown left) is added.

<!-- 8月9日 -->


<div id="outer">

<h3 id="title-1">2012/08/09</h3> <img src="http://openwetware.org/images/thumb/e/e3/20120809_%E2%91%A1.jpg/800px-20120809_%E2%91%A1.jpg" width="620px"><br>

<div id="kind">10% acrylamide gel</div><br> <div id="ul"> condition <ul> <li>We use 10% and 20 % acrylamide gel for the erectrophoresis.</li> <li>We stained the 10% gel with midori green, but the gel wasn't stained clearly(the picture is not here).</li> <li>The above picture is the 20% gel stained with sybr gold.</li> </ul> result <ul> <li>Each Selector seemed to hybridize with the target.</li> </ul> </div>

</div>

<!-- 8月17日 --> <div id="outer"> <h3 id="title-1">2012/08/17</h3> <img src="http://openwetware.org/images/thumb/e/e3/20120817_%E2%91%A1.jpg/800px-20120817_%E2%91%A1.jpg" width="620px"><br> <div id="kind">10% acrylamide gel</div> <br> <div id="ul"> condition <ul> <li>In order to get the target to move between Selectors, we waited 50 minutes before the next step.</li> 

</ul>

result <ul> 

 <li>The materials diffused and the bands weren't clear, because we forgot to put the 15μl buffer of 1.25 % concentration of Mg2+ to samples.</li>
 </ul>

</div> </div>

<!-- 8月20日 -->

<div id="outer"> <h3 id="title-1">2012/08/20</h3> <img src="http://openwetware.org/images/thumb/5/5d/20120820_10-_%E2%91%A1.jpg/800px-20120820_10-_%E2%91%A1.jpg" width="620px"><br> <div id="kind">10% acrylamide gel</div> <br>

<img src="http://openwetware.org/images/thumb/a/af/201120820_20-_%E2%91%A1.jpg/767px-201120820_20-_%E2%91%A1.jpg" width="620px"><br> <div id="kind">20% acrylamide gel</div><br> <div id="ul"> condition <ul> <li>To get the target to move between Selectors, we waited one hour before the next step today. We did electrophoresis at 50V in a fridge (at 4 ℃).</li> <li>We stain the 10% gel with sybr gold.</li> <li>The bands in the 20% gel were warped, and we couldn't get any sufficient result.</li> <li>The following is about the electrophoresis of the 10% gel.</li> </ul> result <ul> <li>The target hybridized with Selector 2 in the lane of the target, Selector 1, and 2. The target also hybridized with Selector 3 in the lane of the target, Selector 1, 2, and 3.</li> <li>From this result, it's possible that Target DNA moved from Selector 1 to Selecor 2, and from Selector 2 to Selector 3.</li> <li>However, in the lane of the target, Selector 1 and 2, the band of the target and Selector1 was also clear. So we cannot decide the target actually moved from Selector 1 to Selector 2.</li> <li>In the lane of the target, Selector 1, 2, and 3, no band is around the place of single-stranded Selector 1. Probably we forgot to put Selector 1.</li> <li>In the lane of the target, Selector 1, 2, and 3, no band is around the place of single-stranded Selector 1. Probably we forgot to put Selector 1.</li> <li>Today we don't make sure the target moved from Selector 1 to Selector 2. But it's likely the target moved from Selector 2 to Selector 3.</li> </ul> </div> </div>


<!-- 8月21日 -->

<div id="outer">

<hr> <p> <h3 id="title-1">2012/8/21</h3> <img src="http://openwetware.org/images/thumb/b/be/20120821_%E2%91%A1.jpg/800px-20120821_%E2%91%A1.jpg" width="620px"><br> <div id="kind">10% acrylamide gel</div><br> <div id="ul"> condition <ul> <li>We did electrophoresis with the same condition as yesterday, except that we didn't prepare the lane of the target, Selector1, 2, and 3</li> </ul> result <ul> <li>As a result, in the lane of the target, Selector 1, and 2, the band of Selector 1 cannot be seen. And the band of Selector 2 is seen at the same place as when it was single-stranded</li> 

 <li>The target didn't seem to attach either to Selector 1 or 2</li>

<li>So the Selector 1 and 2 aggregated with themselves or each other</li> <li>As the 20% gel is difficult to get the result and it takes a long time to, We'll use only the 10% gel from now on</li> 

</ul>

</div> </p> <hr>

</div> <!-- 8月22日 -->

<div id="outer"> <h3 id="title-1">2012/08/22</h3> <img src="http://openwetware.org/images/thumb/e/e3/20120822_%E2%91%A1.jpg/800px-20120822_%E2%91%A1.jpg" width="620px"> <div id="kind">10% acrylamide gel</div> <br> <div id="ul"> condition <ul> <li>the voltage of electrophoresis is 50v by constant voltage</li> <li>We used 1X TAE added to Mg2+ as a buffer</li> <li>The room temperature is 28℃</li> <li>We did the electrophoresis for 3 hours</li> <li>We used SYBR Gold as a stain for 10 minutes</li> </ul> result <ul> <li>There were bands of sample mixed target and Selector 1, and target and Selector 2, hybridized each other</li> <li>In sample mixed Selector 1 and Selector 3, there were both bands Selector 1 only and Selector 3 only so Selector 1 and Selector 3 don't interact each other</li> <li>In sample mixed Selector 2 and Selector 3, We could see a black band at one place</li> <li>Maybe Selector 2's hairpin structure and Selector 3's interacted each other.</li> </ul> </div> </div>

<!-- 8月24日 -->

<div id="outer"> <h3 id="title-1">2012/08/24</h3> <img src="http://openwetware.org/images/thumb/a/af/20120824_%E2%91%A1.jpg/791px-20120824_%E2%91%A1.jpg" width="620px"> <div id="kind">10% acrylamide gel</div> <br> <div id="ul"> condition <ul> <li>the voltage of electrophoresis is 50v by constant voltage</li> <li>We used 1X TAE was added to Mg2+ as a buffer</li> <li>The room temperature is 28℃</li> <li>We did the electrophoresis for 3 hours</li> <li>We used SYBR Gold as a stain for 10 minutes</li> </ul> result <ul> <li>Selector 1 seem to hybridised with itself</li> </ul> </div> </div>

<!-- 8月28日 -->

<div id="outer"> <h3 id="title-1">2012/08/28</h3> <img src="http://openwetware.org/images/thumb/5/5a/20120828_%E2%91%A1.jpg/800px-20120828_%E2%91%A1.jpg" width="620px"> <div id="kind">10% acrylamide gel</div> <br> <div id="ul"> condition <ul> <li>the voltage of electrophoresis is cv 50v</li> <li>We used 1X TAE was added to Mg2+ as a buffer</li> <li>The room temperature is 33℃</li> <li>We did the electrophoresis for 3 hours</li> <li>We used SYBR Gold as a stain for 10 minutes</li> </ul> result <ul> <li>We used new base sequence DNA and Selector 1 didn't hybridize with target, but other results were what we want</li> <li> Tm of Selector 1 is lower than today's room temperature, so didn't hybridize</li> <li>Tomorrow we will try in the refrigerator</li> </ul> </div>

</div>


<!-- 8月29日 --> <div id="outer"> <h3 id="title-1">2012/08/29</h3> <img src="http://openwetware.org/images/thumb/4/4f/20120829_%E2%91%A1.jpg/800px-20120829_%E2%91%A1.jpg" width="620px"> <div id="kind">10% acrylamide gel</div><br> <div id="ul"> condition <ul> <li>We used 1X TAE was added to Mg2+ as a buffer</li> <li>The room temperature is 33℃(4℃ in the refrigerator)</li> <li>We did the electrophoresis for 3.5 hours</li> <li>We used SYBR Gold as a stain for 10 minutes</li> </ul> result <ul> <li>Selector 1 didn't hybridize with target. Other samples worked well</li> <li>We will try again with cool buffer</li> </ul> </div>

</div>

<!-- 8月30日 --> <div id="outer"> <h3 id="title-1">2012/08/30</h3> <img src="http://openwetware.org/images/thumb/c/c7/20120830_%E2%91%A1.jpg/800px-20120830_%E2%91%A1.jpg" width="620px"> <div id="kind">10% acrylamide gel</div><br> <div id="ul"> condition <ul> <li>We used 1X TAE was added to Mg2+ as a buffer</li> <li>The room temperature is 33℃(4℃ in the refrigerator)</li> <li>We did the electrophoresis for 3.5 hours in the refrigerator</li> <li>We used SYBR Gold as a stain for 10 minutes</li> </ul> result <ul> <li>The result was what we want</li> <li>Swlwctor 1 hybridized with target</li> </ul> </div> </div>

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