-80 Glycerol Stocks

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(New page: ===Protocol=== *add 1 equivalent of 50% glycerol to overnight stock *add 300μL mixture to each barcode tube *scan each tube on Simple Scan 1000 (on the computer next to the robot) -enter...)
Current revision (13:53, 9 June 2010) (view source)
(Protocol)
 
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===Protocol===
===Protocol===
-
*add 1 equivalent of 50% glycerol to overnight  stock
+
*note: only make '''ONE''' -80 tube for each sample
-
*add 300μL mixture to each barcode tube
+
*mix 150μL of your freshly grown culture with 150μL of 50% glycerol in a barcode tube
*scan each tube on Simple Scan 1000 (on the computer next to the robot)
*scan each tube on Simple Scan 1000 (on the computer next to the robot)
-
-enter data, and click save
+
**enter data, and click save
-
*log position and data into [https://spreadsheets.google.com/ccc?key=0AllHPRPS0Xc2dG1xZV9ud2hPTzVRTXhoTG5YWjh5SXc&hl=en#gid=2 google docs file]
+
*log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file]
*store in -80
*store in -80

Current revision

Protocol

  • note: only make ONE -80 tube for each sample
  • mix 150μL of your freshly grown culture with 150μL of 50% glycerol in a barcode tube
  • scan each tube on Simple Scan 1000 (on the computer next to the robot)
    • enter data, and click save
  • log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file]
  • store in -80
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