-80 Glycerol Stocks
From OpenWetWare
(Difference between revisions)
(New page: ===Protocol=== *add 1 equivalent of 50% glycerol to overnight stock *add 300μL mixture to each barcode tube *scan each tube on Simple Scan 1000 (on the computer next to the robot) -enter...) |
Current revision (13:53, 9 June 2010) (view source) (→Protocol) |
||
| (3 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
===Protocol=== | ===Protocol=== | ||
| - | * | + | *note: only make '''ONE''' -80 tube for each sample |
| - | + | *mix 150μL of your freshly grown culture with 150μL of 50% glycerol in a barcode tube | |
*scan each tube on Simple Scan 1000 (on the computer next to the robot) | *scan each tube on Simple Scan 1000 (on the computer next to the robot) | ||
| - | + | **enter data, and click save | |
| - | *log position and data into [ | + | *log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file] |
*store in -80 | *store in -80 | ||
Current revision
Protocol
- note: only make ONE -80 tube for each sample
- mix 150μL of your freshly grown culture with 150μL of 50% glycerol in a barcode tube
- scan each tube on Simple Scan 1000 (on the computer next to the robot)
- enter data, and click save
- log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file]
- store in -80
