-80 Glycerol Stocks: Difference between revisions
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===Protocol=== | ===Protocol=== | ||
* | *note: only make '''ONE''' -80 tube for each sample | ||
*mix 150μL of your freshly grown culture with 150μL of 50% glycerol in a barcode tube | |||
*scan each tube on Simple Scan 1000 (on the computer next to the robot) | *scan each tube on Simple Scan 1000 (on the computer next to the robot) | ||
**enter data, and click save | **enter data, and click save | ||
*log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file] | *log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file] | ||
*store in -80 | *store in -80 | ||
Latest revision as of 10:53, 9 June 2010
Protocol
- note: only make ONE -80 tube for each sample
- mix 150μL of your freshly grown culture with 150μL of 50% glycerol in a barcode tube
- scan each tube on Simple Scan 1000 (on the computer next to the robot)
- enter data, and click save
- log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file]
- store in -80
