-80 Glycerol Stocks

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Protocol

  • add 1 equivalent of 50% glycerol to overnight stock
  • add 300μL mixture to each barcode tube
  • scan each tube on Simple Scan 1000 (on the computer next to the robot)

--enter data, and click save

  • log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file]
  • store in -80