20.109(F07): ECD experimental variations: Difference between revisions

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Revision as of 08:56, 25 November 2007


20.109(F07): Laboratory Fundamentals of Biological Engineering

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Since you are the first class to build electrochromic devices with bacteriophage, there are many variations to the protocols that may improve the biomaterials produced. This page should be used to document the experimental choices you've made along the way and their outcome. The collected wisdom that results will be used to make the experiment more robust for future researchers and may lead to alternative methods that could be explored. Thanks for doing experiments that haven't been done before and for letting everyone know how they work.

T/R lab

team name Iridium Chloride pH PFU/ul reacted with Iridium number of electrical pulses time, temp, shaking for growing nanowires comments on TEM data
TRpink 7.00 3x10^11 PFU/mL 35
TRyellow
TRblue
TRpurple 7.45
TRred 7.44 2.4E8 30 2hrs, rm temp
TRgreen 7.09 45 2hrs, room temp

W/F lab

team name Iridium Chloride pH PFU/ul reacted with Iridium number of electrical pulses time, temp, shaking for growing nanowires comments on TEM data
WFpink
WFyellow
WFblue 7.22 3.9*10^7
WFpurple
WFred 7.00 2.8e6 (used 80ul instead of 40ul) 35 2hr, RT
WFgreen 7.1 7.6e6 (used 53μL to increase ratio) 45/50 1hr 54min, room T