20.109(F07): Genome engineering assessment

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20.109(F07): Laboratory Fundamentals of Biological Engineering

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Portfolio components

Part 1:Rebuttal to editorial

This will be written as a homework assignment, exchanged with your lab partner for peer review, then submitted to the teaching faculty as part of your portfolio

Part 2: M13.1 redesign description and parts

Part 3: Data summary for p3-modifications you performed in lab

You'll find guidelines for writing here. Additionally, many of the "for next time" assignments can get you started on this part of the portfolio. Including but not limited to:

  • Oligonucleotide design, sequence consequences for phage when inserted and sequence data
  • Ligation results (table)
  • Agarose gel examining candidate clones (figure)
  • Western results (figure)
  • Plaque assay (figure)
  • Short paragraph for each table and figure describing and interpreting what's shown
  • One or two sentence summary of your experimental results
  • One or two sentence proposal for what you'd do next if we had one more month to spend on this project

Part 4: Registry of Standard Biological Parts

  • Existing strengths/usefulness
  • Measures for success of registry
  • Suggestions for improvement