20.109(F07): Journal article discussion

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20.109(F07): Laboratory Fundamentals of Biological Engineering

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Journal articles to be discussed

  • The news story by Erika Check called "RNA interference: hitting the on switch" published in Nature 2007 vol. 448 pp. 855-8
  • The original paper by Long-Cheng Li et. al. "Small dsRNAs induce transcriptional activation in human cells" published in PNAS 2006 vol. 103 pp. 17337-42.

Questions to guide your reading

Read the news story by Erika Check first, noting particularly the reporters description of the motivation and path to RNA activation. You should compare this to how the scientific authors describe their path to RNAa in the introduction to the paper they wrote in their paper.

For next time

  1. Calculate the volume for 4 ug, 2 ug, 1 ug and 0.5 ug of each RNA sample that you prepared. Show your work. Save a copy of your answer since you'll need to know this volume to perform your experiment.
  2. Please familiarize yourself with the basics of microarrays by reading NCBI's primer on the technique.
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