20.109(F08): Fall 2008 schedule: Difference between revisions

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| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
| [[20.109(F08): Mod 1 Day 4 DNA ligations and bacterial transformations| DNA ligation and bacterial transformation]]
| [[20.109(F08): Mod 1 Day 4 DNA ligations and bacterial transformations| DNA ligation and bacterial transformation]]
| [[Media:MOD1DAY4.pdf| NK lab notes]]
| [[Media:MOD1DAY4.pdf| NK lab notes]], [[Media:F08_M1D4-ANS.pdf| ANS lab notes]]
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| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
| [[20.109(F08): Mod 1 Day 5 Examine candidate clones| Examine candidate clones]]
| [[20.109(F08): Mod 1 Day 5 Examine candidate clones| Examine candidate clones]]
|  
| [[Media:MOD1DAY5.pdf| NK lab notes]], [[Media:F08_M1D5-ANS.pdf| ANS lab notes]]
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| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
| [[20.109(F08): Mod 1 Day 6 Restriction map and tissue culture | Restriction map and tissue culture]]
| [[20.109(F08): Mod 1 Day 6 Restriction map and tissue culture | Restriction map and tissue culture]]
|  
| [[Media:Mod1day6.ppt| NK lab notes]], [[Media:F08_M1D6-ANS.pdf| ANS lab notes]]
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| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
| [[20.109(F08): Mod 1 Day 7 Lipofection| Lipofection]]
| [[20.109(F08): Mod 1 Day 7 Lipofection| Lipofection]]
| your first draft of the [[20.109(F08):DNA engineering lab report guidelines| Mod 1 lab report]] is due before lab today
| your first draft of the [[20.109(F08):DNA engineering lab report guidelines| Mod 1 lab report]] is due before lab today<br>
[[Media:F08_M1D7-ANS.pdf| ANS lab notes]]
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| [http://openwetware.org/wiki/User:Brian_Belmont BB]
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
| [[20.109(F08): Mod 2 Day 1 Protein engineering with PCR| Protein engineering with PCR]]
| [[20.109(F08): Mod 2 Day 1 Protein engineering with PCR| Protein engineering with PCR]]
| your [[20.109(F08):DNA engineering powerpoint pitch guidelines| PowerPoint Pitch]] is due before lab today
| your [[20.109(F08):DNA engineering powerpoint pitch guidelines| PowerPoint Pitch]] is due before lab today<br>[[Media:20.109(F08) handoutM2D1.pdf| M2D1 lecture notes]]<br>[[Media:MOD2DAY1_wiki.ppt.pptx| NK lab notes]], [[Media:F08_M2D1-ANS.pdf| ANS lab notes]]
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| [http://openwetware.org/wiki/User:Brian_Belmont BB]
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
| [[20.109(F08): Mod 2 Day 2 Yeast transformation| Yeast transformation]]
| [[20.109(F08): Mod 2 Day 2 Yeast transformation| Yeast transformation]]
|  
| [[Media:20.109(F08) handout M2D2.pdf| M2D2 lecture notes]]<br>[[Media:MOD2DAY2_wiki.ppt| NK lab notes]], [[Media:F08_M2D2-ANS.pdf| ANS lab notes]]
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| [http://openwetware.org/wiki/User:Brian_Belmont BB]
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
| [[20.109(F08): Mod 2 Day 3 Colony PCR and journal article discussion| Colony PCR, Journal article discussion]]
| [[20.109(F08): Mod 2 Day 3 Colony PCR and journal article discussion| Colony PCR, Journal article discussion]]
|
| [[Media:20.109(F08) handout M2D3.pdf| M2D3 lecture notes]]<br>[[Media:MOD2DAY3_wiki.ppt| NK lab notes]], [[Media:F08_M2D3-ANS.pdf| ANS lab notes]]
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| [http://openwetware.org/wiki/User:Brian_Belmont BB]
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
| [[20.109(F08): Mod 2 Day 4 SDS-PAGE, screen for phenotypes| SDS-PAGE, screen for phenotypes]]
| [[20.109(F08): Mod 2 Day 4 SDS-PAGE, screen for phenotypes| SDS-PAGE, screen for phenotypes]]
|
| [[Media:20.109(F08) handout M2D4.pdf| M2D4 lecture notes]] <br>[[Media:MOD2DAY4_wiki.ppt| NK lab notes]], [[Media:F08_M2D4-ANS.pdf| ANS lab notes]]
 
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| [http://openwetware.org/wiki/User:Brian_Belmont BB]
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
| [[20.109(F08): Mod 2 Day 5 Probe western, isolate RNA| Probe Western, isolate RNA]]
| [[20.109(F08): Mod 2 Day 5 Probe western, isolate RNA| Probe Western, isolate RNA]]
| guest lecture by [http://web.mit.edu/be/people/white.htm Prof. Forest White] about mass spec analysis of protein complexes
| guest lecture by [http://web.mit.edu/be/people/white.htm Prof. Forest White] about mass spec analysis of protein complexes. His slides are [[Media:20 109 MS lecture.ppt| here]]<br>[[Media:MOD2DAY5_wiki.ppt| NK lab notes]], [[Media:F08_M2D5-ANS.pdf| ANS lab notes]]
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| [http://openwetware.org/wiki/User:Brian_Belmont BB]
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
| [[20.109(F08): Mod 2 Day 7 cDNA synthesis and microarray| cDNA synthesis and microarray]]
| [[20.109(F08): Mod 2 Day 7 cDNA synthesis and microarray| cDNA synthesis and microarray]]
|  
| [[Media:20.109(F08) M2D7 handout.pdf| M2D7 lecture notes]]<br>[[Media:MOD2DAY7_wiki.ppt| NK lab notes]], [[Media:F08_M2D7-ANS.pdf| ANS lab notes]]
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| [http://openwetware.org/wiki/Luis_Alvarez LA]
| [http://openwetware.org/wiki/Luis_Alvarez LA]
| [[20.109(F08): Mod 3 Day 1 Growth of phage materials| Phage growth for materials]]
| [[20.109(F08): Mod 3 Day 1 Growth of phage materials| Phage growth for materials]]
| your first draft of your research article is due before lab today
| your first draft of your research article is due before lab today<br>[[Media:Mod3Day1.ppt| NK lab notes]], [[Media:F08_M3D1-ANS.pdf| ANS lab notes]]
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| [http://openwetware.org/wiki/Luis_Alvarez LA]
| [http://openwetware.org/wiki/Luis_Alvarez LA]
| [[20.109(F08): Mod 3 Day 2 Phage nanowires| Phage nanowires]]
| [[20.109(F08): Mod 3 Day 2 Phage nanowires| Phage nanowires]]
|  
| [[Media:Mod3Day2.ppt| NK lab notes]], [[Media:F08_M3D2-ANS.pdf| ANS lab notes]]
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| colspan="7" |
| colspan="7" |
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| [http://openwetware.org/wiki/Luis_Alvarez LA]
| [http://openwetware.org/wiki/Luis_Alvarez LA]
| [[20.109(F08): Mod 3 Day 4 Phage by design| Phage by design]]
| [[20.109(F08): Mod 3 Day 4 Phage by design| Phage by design]]
|  
| [[Media:Mod3Day4.ppt| NK lab notes]], [[Media:F08_M3D4-ANS.pdf| ANS lab notes]]
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| [http://openwetware.org/wiki/Luis_Alvarez LA]
| [http://openwetware.org/wiki/Luis_Alvarez LA]
| [[20.109(F08): Mod 3 Day 5 Phage by design, pt2| Phage by design, pt 2]]
| [[20.109(F08): Mod 3 Day 5 Phage by design, pt2| Phage by design, pt 2]]
|  
| [[Media:Mod3Day5.ppt| NK lab notes]], [[Media:F08_M3D4-ANS.pdf| ANS lab notes]]
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| colspan="7" |
| colspan="7" |
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| [http://openwetware.org/wiki/Luis_Alvarez LA]
| [http://openwetware.org/wiki/Luis_Alvarez LA]
| [[20.109(F08): Mod 3 Day 6 ECD assembly| ECD assembly]]
| [[20.109(F08): Mod 3 Day 6 ECD assembly| ECD assembly]]
|  
| [[Media:Mod3Day6.ppt| NK lab notes]], [[Media:F08_M3D6-ANS.pdf| ANS lab notes]]
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Latest revision as of 08:27, 5 December 2008


20.109(F08): Laboratory Fundamentals of Biological Engineering

Home        People        Schedule Fall 2008        Assignments        Lab Basics        OWW Basics       
DNA Engineering        Protein Engineering        Biomaterials Engineering              

MODULE DAY DATE LECTURER TA LAB note
Thur Sept 4 NK Orientation orientation day handout
1 1 Tue Sept 9 BE MS DNA engineering using PCR NK lab notes, ANS lab notes
1 2 Thur Sept 11 BE MS Clean and cut DNA NK lab notes, ANS lab notes
1 3 Tue Sept 16 BE MS Agarose gel electrophoresis NK lab notes, ANS lab notes
1 4 Thur Sept 18 BE MS DNA ligation and bacterial transformation NK lab notes, ANS lab notes
1 5 Tue Sept 23 BE MS Examine candidate clones NK lab notes, ANS lab notes
1 6 Thur Sept 25 BE MS Restriction map and tissue culture NK lab notes, ANS lab notes
1 7 Tue Sept 30 BE MS Lipofection your first draft of the Mod 1 lab report is due before lab today

ANS lab notes

1 8 Thur Oct 2 BE MS FACS analysis
2 1 Tues Oct 7 NK BB Protein engineering with PCR your PowerPoint Pitch is due before lab today
M2D1 lecture notes
NK lab notes, ANS lab notes
2 2 Thur Oct 9 NK BB Yeast transformation M2D2 lecture notes
NK lab notes, ANS lab notes
2 3 Tues Oct 14 NK BB Colony PCR, Journal article discussion M2D3 lecture notes
NK lab notes, ANS lab notes
2 4 Thur Oct 16 NK BB SDS-PAGE, screen for phenotypes M2D4 lecture notes
NK lab notes, ANS lab notes
2 5 Tues Oct 21 NK BB Probe Western, isolate RNA guest lecture by Prof. Forest White about mass spec analysis of protein complexes. His slides are here
NK lab notes, ANS lab notes
2 6 Thurs Oct 23 NK BB Journal Club I optional visit to Abcam during lecture hour
2 7 Tues Oct 28 NK BB cDNA synthesis and microarray M2D7 lecture notes
NK lab notes, ANS lab notes
2 8 Thurs Oct 30 NK BB Microarray data analysis
3 1 Tues Nov 4 AB LA Phage growth for materials your first draft of your research article is due before lab today
NK lab notes, ANS lab notes
3 2 Thurs Nov 6 AB LA Phage nanowires NK lab notes, ANS lab notes
Veteran's Day Break Nov 10,11 No lab Tuesday and Wednesday
3 3 Thur Nov 13 AB LA Journal Club II
3 4 Tue Nov 18 AB LA Phage by design NK lab notes, ANS lab notes
3 5 Thur Nov 20 AB LA Phage by design, pt 2 NK lab notes, ANS lab notes
Thanksgiving Break Nov No lab or lecture all week
3 6 Tue Dec 2 AB LA ECD assembly NK lab notes, ANS lab notes
3 7 Thur Dec 4 AB LA Oral presentations
3 8 Tue Dec 9 end of term celebration... Lecture for class evaluations then lunch from noon-1:30 PM, no lab
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