20.109(F08): The grafting parlour: Difference between revisions
No edit summary |
|||
(8 intermediate revisions by the same user not shown) | |||
Line 41: | Line 41: | ||
from Lucy 12.08.08 | from Lucy 12.08.08 | ||
*There are ways to convert a webcam into a microscope [http://www.engadget.com/2007/12/17/build-a-digital-microscope-from-a-webcam/] but my guess is that the magnification is not great. | *There are ways to convert a webcam into a microscope [http://www.engadget.com/2007/12/17/build-a-digital-microscope-from-a-webcam/] but my guess is that the magnification is not great. | ||
* | *Whether microscope or document camera, it depends on how wide a view will be captured. Here is | ||
one that works with existing analog microscopes: [http://www.bodelin.com/proscopehr/] | one that works with existing analog microscopes: [http://www.bodelin.com/proscopehr/] | ||
*And here is more information on the document camera that I've been looking | *And here is more information on the document camera that I've been looking | ||
at: [http://www.everythingusb.com/avermedia_avervision_150.html] | at: [http://www.everythingusb.com/avermedia_avervision_150.html] | ||
=G1 to S transition= | |||
==Primers to check Cln1 and Cln2:GFP strains== | ==Primers to check Cln1:GFP and Cln2:GFP strains== | ||
===Cln1=== | From intro of [http://www.nature.com/msb/journal/v5/n1/full/msb200978.html Fred Cross paper on Clb2]: "Cyclins and cyclin-dependent kinase (Cdk) provide excellent candidates for a central controlling timer. In all eukaryotes, mitotic cyclin–Cdk activity rises and falls once per division. Mitotic cyclins (CLB1, 2, 3, and 4 in S. cerevisiae) are required for mitotic entry (spindle assembly and anaphase). However, overexpression of mitotic cyclin prevents mitotic exit (spindle disassembly and cytokinesis), resulting in telophase arrest (Surana et al, 1993 [[PMID: 8491189]])." So perhaps a system in which CLN1:GFP fusion is controlled on a plasmid and is Gal inducible might arrest at S? | ||
Forward: CCCT TTTCTCTCTATGCCCAT | ===[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=CLN1 Cln1]= 1641bp=== | ||
[[Image:CLN1 fwd.png|thumb|left| [http://www.ncbi.nlm.nih.gov/blast/bl2seq/wblast2.cgi BLAST2] seq for fwd primer and CLN1]] | |||
[[Image:CLN1 rev.png|thumb|left| [http://www.ncbi.nlm.nih.gov/blast/bl2seq/wblast2.cgi BLAST2] seq for rev primer and CLN1+500bp(3')]]<br> | |||
NO270=Forward: CCCT TTTCTCTCTATGCCCAT | |||
*length = 21 | *length = 21 | ||
*Tm = | *Tm = 54.3 | ||
*GC = 47.6 | *GC = 47.6 | ||
Reverse: CTAG ATGTTTGTAGGTGGGCA | NO271=Reverse: CTAG ATGTTTGTAGGTGGGCA | ||
*length = 21 | *length = 21 | ||
*Tm = | *Tm = 54.3 | ||
*GC = 47.6 | *GC = 47.6 | ||
PCR product = 977bp<br> | PCR product = 977bp<br> | ||
PCR product+GFP = 1690bp | PCR product+GFP = 1690bp<br><br><br> | ||
===Cln2=== | ===[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=CLN2 Cln2] = 1638 bp=== | ||
Forward: CACGGCATATTCTCCATTATC | [[Image:CLN2 fwd.png|thumb|left| [http://www.ncbi.nlm.nih.gov/blast/bl2seq/wblast2.cgi BLAST2] seq for fwd primer and CLN2]] | ||
[[Image:CLN2 rev.png|thumb|left| [http://www.ncbi.nlm.nih.gov/blast/bl2seq/wblast2.cgi BLAST2] seq for rev primer and CLN2+500bp(3')]]<br> | |||
NO272= Forward: CACGGCATATTCTCCATTATC | |||
*length: 21 | *length: 21 | ||
*Tm = | *Tm = 50.9 | ||
*GC = 42.9 | *GC = 42.9 | ||
Reverse: GGTCTCTTTTTGGTACGTTTG | NO273= Reverse: GGTCTCTTTTTGGTACGTTTG | ||
*length = 21 | *length = 21 | ||
*Tm = | *Tm = 51.8 | ||
*GC = 42.9 | *GC = 42.9 | ||
PCR product = 804bp<br> | PCR product = 804bp<br> | ||
PCR product+GFP = 1517bp | PCR product+GFP = 1517bp | ||
===Additional Primers=== | |||
<center> | |||
{| border="1" | |||
! NO# | |||
| Name | |||
| Seq | |||
| Length, adds | |||
| Tm | |||
| G/C | |||
|- | |||
| NO274 | |||
| CLN1_2GFP_-900fwd | |||
| CATTAG GCCGGC ACAGCATTC CCTTGTTCGC AACACTT | |||
| 26, Random NaeI cln1 ~900bp before | |||
| 63.4 | |||
| 46.2 | |||
|- | |||
| NO275 | |||
| CLN1_2GFP_rev | |||
| CATTAG GGATCC CAGTTGA GAGCTATTGT GGTTCCTTA | |||
| 26, Random BamHI cln1 | |||
| 63.0 | |||
| 42.3 | |||
|- | |||
| NO276 | |||
| CLN2_2GFP_-300forward | |||
| CATTAG GGTACC GCAGCC TCTGGCTACT TGTTTAACTT | |||
| 26, Random KpnI cln2 ~300 bp before | |||
| 63.4 | |||
| 46.2 | |||
|- | |||
| NO277 | |||
| CLN2_2GFP_rev | |||
| CATTAG GGATCC TACTTGGGTA TTGCCCATACCA AAAG | |||
| 26, Random BamHI cln2 | |||
| 63 | |||
| 42.3 | |||
|- | |||
| NO278 | |||
| CLN+GFP_rev | |||
| CATTAG GCATGC TCACTTGTTCAATAGGCCTATGCCAT | |||
| 29, Random SphI GFP | |||
| 63.4 | |||
| 46.2 | |||
|} | |||
</center> | |||
=G2 to M transition= | |||
==[http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=CLB2 Clb2]= 1475bp== | |||
*Reference with Clb2:YFP is [http://www.nature.com/msb/journal/v5/n1/full/msb200978.html here]. This reference also includes (as Fig 1) depiction of CFP-[http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=TUB1 TUB1] to label cytoskeleton blue, as well as [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=MYO1 MYO1]-mCherry to label red the contractile ring from cytokinesis. |
Latest revision as of 11:21, 24 December 2009
NOTES FROM 11.11 meeting with the artist from THE GRAFTING PARLOUR
Please edit and add information that I didn't capture
Nkuldell 16:39, 11 November 2008 (EST)
Initial themes
- Form brings questions about content
- Computational approaches represent nature but biology holds in itself the reality of nature
- Art can tilt and sway perspective
- Interactive technology (video, telecommunication) are time base media
Framing questions
- How to look at the world through nature’s point of view?
- How can artwork change itself during a show?
- How can artwork change as it travels from gallery to gallery?
- We value the history of an object but can an object have traces/memories of itself and its history?
- Is the human desire to “fix time” immutable?
- We think of our cells as making up us but they have a life of their own as well (circadian pulsing of neurons every 23.5 hours w/o stimulus). What is the biological memory that cells have of a day? What do cells have to say to us?
- Galleries usually carefully manage light/moisture/temperature to inhibit bacteria growth on art but is this just a romantic notion of art by masters and is the intervention needed? Can unpredictable evolution/passage/change of art be part of art?
Art/Science examples we considered
- Eduardo Kac
- Transgenetic Alba bunny: use of animals as art? Research was done for science then made accessible through art
- Specimen of Secrecy About Marvelous Discoveries, pt 1
- Specimen of Secrecy About Marvelous Discoveries, pt2
- Hyunkoo Lee: animatus= skeletons from animated characters. Notable for its performance of science, that the artist makes apparent. Some eerie some playful examples.
NOTES: about microscope/display
from Lucy 12.08.08
- There are ways to convert a webcam into a microscope [1] but my guess is that the magnification is not great.
- Whether microscope or document camera, it depends on how wide a view will be captured. Here is
one that works with existing analog microscopes: [2]
- And here is more information on the document camera that I've been looking
at: [3]
G1 to S transition
Primers to check Cln1:GFP and Cln2:GFP strains
From intro of Fred Cross paper on Clb2: "Cyclins and cyclin-dependent kinase (Cdk) provide excellent candidates for a central controlling timer. In all eukaryotes, mitotic cyclin–Cdk activity rises and falls once per division. Mitotic cyclins (CLB1, 2, 3, and 4 in S. cerevisiae) are required for mitotic entry (spindle assembly and anaphase). However, overexpression of mitotic cyclin prevents mitotic exit (spindle disassembly and cytokinesis), resulting in telophase arrest (Surana et al, 1993 PMID: 8491189)." So perhaps a system in which CLN1:GFP fusion is controlled on a plasmid and is Gal inducible might arrest at S?
Cln1= 1641bp
NO270=Forward: CCCT TTTCTCTCTATGCCCAT
- length = 21
- Tm = 54.3
- GC = 47.6
NO271=Reverse: CTAG ATGTTTGTAGGTGGGCA
- length = 21
- Tm = 54.3
- GC = 47.6
PCR product = 977bp
PCR product+GFP = 1690bp
Cln2 = 1638 bp
NO272= Forward: CACGGCATATTCTCCATTATC
- length: 21
- Tm = 50.9
- GC = 42.9
NO273= Reverse: GGTCTCTTTTTGGTACGTTTG
- length = 21
- Tm = 51.8
- GC = 42.9
PCR product = 804bp
PCR product+GFP = 1517bp
Additional Primers
NO# | Name | Seq | Length, adds | Tm | G/C |
---|---|---|---|---|---|
NO274 | CLN1_2GFP_-900fwd | CATTAG GCCGGC ACAGCATTC CCTTGTTCGC AACACTT | 26, Random NaeI cln1 ~900bp before | 63.4 | 46.2 |
NO275 | CLN1_2GFP_rev | CATTAG GGATCC CAGTTGA GAGCTATTGT GGTTCCTTA | 26, Random BamHI cln1 | 63.0 | 42.3 |
NO276 | CLN2_2GFP_-300forward | CATTAG GGTACC GCAGCC TCTGGCTACT TGTTTAACTT | 26, Random KpnI cln2 ~300 bp before | 63.4 | 46.2 |
NO277 | CLN2_2GFP_rev | CATTAG GGATCC TACTTGGGTA TTGCCCATACCA AAAG | 26, Random BamHI cln2 | 63 | 42.3 |
NO278 | CLN+GFP_rev | CATTAG GCATGC TCACTTGTTCAATAGGCCTATGCCAT | 29, Random SphI GFP | 63.4 | 46.2 |