20.109(F08): The grafting parlour

NOTES FROM 11.11 meeting with the artist from THE GRAFTING PARLOUR

Please edit and add information that I didn't capture
Nkuldell 16:39, 11 November 2008 (EST)

Initial themes

• Form brings questions about content
• Computational approaches represent nature but biology holds in itself the reality of nature
• Art can tilt and sway perspective
• Interactive technology (video, telecommunication) are time base media

Framing questions

• How to look at the world through nature’s point of view?
• How can artwork change itself during a show?
• How can artwork change as it travels from gallery to gallery?
• We value the history of an object but can an object have traces/memories of itself and its history?
• Is the human desire to “fix time” immutable?
• We think of our cells as making up us but they have a life of their own as well (circadian pulsing of neurons every 23.5 hours w/o stimulus). What is the biological memory that cells have of a day? What do cells have to say to us?
• Galleries usually carefully manage light/moisture/temperature to inhibit bacteria growth on art but is this just a romantic notion of art by masters and is the intervention needed? Can unpredictable evolution/passage/change of art be part of art?

Art/Science examples we considered

• GFP projects from Marc Zimmer: Static images that were not intended as art
  • pt1
  • Brainbow
  • GFPixle

• Eduardo Kac
  • Transgenetic Alba bunny: use of animals as art? Research was done for science then made accessible through art
  • Specimen of Secrecy About Marvelous Discoveries, pt 1
  • Specimen of Secrecy About Marvelous Discoveries, pt2

• Prof. Eshel Ben-Jacob
  • Prof. images pt 1
  • pdf

• Prof. Micha Spira

• Hyunkoo Lee: animatus= skeletons from animated characters. Notable for its performance of science, that the artist makes apparent. Some eerie some playful examples.

NOTES: about microscope/display

from Lucy 12.08.08

• There are ways to convert a webcam into a microscope [1] but my guess is that the magnification is not great.
• Whether microscope or document camera, it depends on how wide a view will be captured. Here is

one that works with existing analog microscopes: [2]

• And here is more information on the document camera that I've been looking

at: [3]

G1 to S transition

Primers to check Cln1:GFP and Cln2:GFP strains

Cln1= 1641bp

BLAST2 seq for fwd primer and CLN1

BLAST2 seq for rev primer and CLN1+500bp(3')

NO270=Forward: CCCT TTTCTCTCTATGCCCAT

• length = 21
• Tm = 54.3
• GC = 47.6

NO271=Reverse: CTAG ATGTTTGTAGGTGGGCA

• length = 21
• Tm = 54.3
• GC = 47.6

PCR product = 977bp
PCR product+GFP = 1690bp

Cln2 = 1638 bp

BLAST2 seq for fwd primer and CLN2

BLAST2 seq for rev primer and CLN2+500bp(3')

NO272= Forward: CACGGCATATTCTCCATTATC

• length: 21
• Tm = 50.9
• GC = 42.9

NO273= Reverse: GGTCTCTTTTTGGTACGTTTG

• length = 21
• Tm = 51.8
• GC = 42.9

PCR product = 804bp
PCR product+GFP = 1517bp

Additional Primers

NO#	Name	Seq	Length, adds	Tm	G/C
NO274	CLN1_2GFP_-900fwd	CATTAG GCCGGC ACAGCATTC CCTTGTTCGC AACACTT	26, Random NaeI cln1 ~900bp before	63.4	46.2
NO275	CLN1_2GFP_rev	CATTAG GGATCC CAGTTGA GAGCTATTGT GGTTCCTTA	26, Random BamHI cln1	63.0	42.3
NO276	CLN2_2GFP_-300forward	CATTAG GGTACC GCAGCC TCTGGCTACT TGTTTAACTT	26, Random KpnI cln2 ~300 bp before	63.4	46.2
NO277	CLN2_2GFP_rev	CATTAG GGATCC TACTTGGGTA TTGCCCATACCA AAAG	26, Random BamHI cln2	63	42.3
NO278	CLN+GFP_rev	CATTAG GCATGC TCACTTGTTCAATAGGCCTATGCCAT	29, Random SphI GFP	63.4	46.2

G2 to M transition

Clb2= 1475bp

• Reference with Clb2:YFP is here. This reference also includes (as Fig 1) depiction of CFP-TUB1 to label cytoskeleton blue, as well as MYO1-mCherry to label red the contractile ring from cytokinesis.