20.109(F09):System engineering research article guidelines: Difference between revisions
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(New page: == Day 1== In advance of Module: *streak out NB334 onto LB+Amp+Cam+Kan *streak out NB188 onto LB+Amp+Kan In advance of...) |
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*streak out NB334 onto LB+Amp+Cam+Kan | *streak out NB334 onto LB+Amp+Cam+Kan | ||
*streak out NB188 onto LB+Amp+Kan | *streak out NB188 onto LB+Amp+Kan | ||
*streak out NB5 on LB+Amp | |||
In advance of Day 1: | In advance of Day 1: | ||
*Prepare 50 ml bottles of indicator media but don't add H2O | *Prepare 50 ml bottles of indicator media but don't add H2O | ||
*Grow ONs of NB5 in LB+Amp | |||
On lab day: | |||
*In AM subculture NB5, 1 ml/20ml LB+Amp on RT shaker. Induce with IPTG at start of lab period. | |||
Latest revision as of 18:32, 17 August 2009
Day 1
In advance of Module:
- streak out NB334 onto LB+Amp+Cam+Kan
- streak out NB188 onto LB+Amp+Kan
- streak out NB5 on LB+Amp
In advance of Day 1:
- Prepare 50 ml bottles of indicator media but don't add H2O
- Grow ONs of NB5 in LB+Amp
On lab day:
- In AM subculture NB5, 1 ml/20ml LB+Amp on RT shaker. Induce with IPTG at start of lab period.
