20.109(F09): Omar Abudayyeh and Pablo Crespo Research Proposal: Difference between revisions
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==Project Overview== | ==Project Overview== | ||
This project aims to engineer phages that are able to selectively target cancer cells in vitro and deliver cargo that can result in tumor death. Because phages have multiple types of proteins on their outer coat, the phages can be designed to bind to other substrates, such as drugs or gold, in addition to the tumor. This allows for targeted delivery of drugs or for photothermal cancer therapy through irradiation of the gold. | |||
==Background Information== | ==Background Information== | ||
Revision as of 18:35, 19 November 2009
Engineering phages to target tumors
Project Overview
This project aims to engineer phages that are able to selectively target cancer cells in vitro and deliver cargo that can result in tumor death. Because phages have multiple types of proteins on their outer coat, the phages can be designed to bind to other substrates, such as drugs or gold, in addition to the tumor. This allows for targeted delivery of drugs or for photothermal cancer therapy through irradiation of the gold.
Background Information
Research Problem and Goals
Project Methods
Predicted Outcomes
Resources Required
References
- Krag, D., Shukla, G., Shen, G., Pero, S., Ashikaga, T., Fuller, S., Weaver, D., Burdette-Radoux S., & Thomas, C. Selection of tumor-binding ligands in cancer patients with phage display libraries. Cancer Research. 2006; 66: 7724-7733.
- The researchers used phage display libraries to identify ligands that could be used for targeting cancer, including breast, melanoma, and pancreas. Repeated panning was done by allowing phages to hone in on tumors in patients with stage IV cancer and then excising the tumors and recovering phages that bound. The study identified several motifs on the binding peptide indicating nonrandom specificity for tumors.
