20.109(F09): TA's notes for module 3: Difference between revisions
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===Day 1 solutions=== | ===Day 1 solutions=== | ||
*Each group needs an overnight culture of ER2267 grown in LB+Kan | *Each group needs an overnight culture of ER2267 grown in LB+Kan | ||
*Each group needs 4 LB plates | |||
*Each group needs 12 ml Top Agar | |||
*Each group needs 50 ml of infected XL1-blue | |||
'''CTAB''' | '''CTAB''' | ||
*Each group should make a 0.1 M solution of hexadecyltrimethylammonium bromide ("CTAB"). This is done by mixing 0.72 g with 20 ml of water in a 50 ml conical tube. | *Each group should make a 0.1 M solution of hexadecyltrimethylammonium bromide ("CTAB"). This is done by mixing 0.72 g with 20 ml of water in a 50 ml conical tube. | ||
Revision as of 04:19, 20 September 2009
Day 1 solutions
- Each group needs an overnight culture of ER2267 grown in LB+Kan
- Each group needs 4 LB plates
- Each group needs 12 ml Top Agar
- Each group needs 50 ml of infected XL1-blue
CTAB
- Each group should make a 0.1 M solution of hexadecyltrimethylammonium bromide ("CTAB"). This is done by mixing 0.72 g with 20 ml of water in a 50 ml conical tube.
Solutions to share
- One group will be responsible for making each of the following solutions that the class will use in the synthesis of nanowires.
- Pool the volume you make with a second group making the same solution if there is another group assigned this task.
- 10 mM hydrated hydrogen tetrachloroaurate (HAuCl4•3H2O) = Mix 40 mg with 10 mL water
- 10 mM silver nitrate (AgNO3)= Mix 17 mg with 10 mL water
- 0.1 M ascorbic acid = Mix 0.18 gram with 10 mL water
- 2.5 M NaCl = Mix 7.3 g NaCl with 50 ml water
