20.109(F09): TA notes for module 2: Difference between revisions

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==[[20.109(F09): Mod 2 Day 1 Testing an engineered biological system| Day 1]]==
==[[20.109(F09): Mod 2 Day 1 Testing an engineered biological system| Day 1]]==
* Test in Solid Media
* Test in Liquid Media
* Practice b-gal
==[[20.109(F09): Mod 2 Day 2 Measuring system performance| Day 2]]==
==[[20.109(F09): Mod 2 Day 2 Measuring system performance| Day 2]]==
* Light/Dark b-gal
* Bacterial Photograph
* Oral Presentation Instruction in lab
==[[20.109(F09): Mod 2 Day 3 Tools for system engineering| Day 3]]==
==[[20.109(F09): Mod 2 Day 3 Tools for system engineering| Day 3]]==
* Transform Library
* Registry of Std Biological Parts
* Electronics
==[[20.109(F09): Journal Club I| Day 4]]==
==[[20.109(F09): Journal Club I| Day 4]]==
* MUG to identify candidates
**Restreak and set up ONs
==[[20.109(F09): Mod 2 Day 5 Assessing re-tuned system| Day 5]]==
==[[20.109(F09): Mod 2 Day 5 Assessing re-tuned system| Day 5]]==
*Protein Gel
*Miniprep/Digest/Agarose gel/Send to Sequence
*Set up Dark/Light ONs
==[[20.109(F09): Mod 2 Day 6 Readouts of DNA, Protein| Day 6]]==
==[[20.109(F09): Mod 2 Day 6 Readouts of DNA, Protein| Day 6]]==
*Probe Western
*b-gal
*Seq analysis
==[[20.109(F09): Journal Club II| Day 7]]==
==[[20.109(F09): Journal Club II| Day 7]]==
*"only" Journal Club

Revision as of 18:57, 24 August 2009

Day 1

  • Test in Solid Media
  • Test in Liquid Media
  • Practice b-gal

Day 2

  • Light/Dark b-gal
  • Bacterial Photograph
  • Oral Presentation Instruction in lab

Day 3

  • Transform Library
  • Registry of Std Biological Parts
  • Electronics

Day 4

  • MUG to identify candidates
    • Restreak and set up ONs

Day 5

  • Protein Gel
  • Miniprep/Digest/Agarose gel/Send to Sequence
  • Set up Dark/Light ONs

Day 6

  • Probe Western
  • b-gal
  • Seq analysis

Day 7

  • "only" Journal Club