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==Announcements==
==Announcements==
*Newest sequencing results for T/R lab using primers NO282, 283, and 296 are available from Genewiz (look at order # 10-180371918). Reactions using the newest primer (NO297) will be sent on Monday and should be ready for you to look at on Tuesday.
*Blogs for Mod3 due Thursday/Friday!!
*Extra office hours with Linda Sutliff are posted to [[User:Linda L. Sutliff| her user page.]] Drop in or schedule a time--but try to take advantage of this resource!
*Re-write grades are posted to Stellar Site. Overall the papers were much improved...congrats to you all!
*The [[Talk:20.109%28F11%29:_Mod_2_Day_6_Readouts_of_DNA%2C_Protein | M2D6 Talk]] page has a place to share your data in summary form. By sharing, we can look for trends in the class results.
 
*FINISHING UP MODULE 1: Your ppts should be back from Bevin at the start of next week, 11.7.11!
*Agi's Monday office hour is cancelled. I'm in no shape to be breathing near anyone; I'm happy to discuss research proposal questions over email instead.
*Agi will hold office hours Monday and Thursday of next week from 2-3:30 pm in room 16-336.
 
*NK will hold office hours on Sunday 4PM in lab and on Thursday 11/10 at 11AM in lab.
*Final TEM update! W/F images have joined T/R at the [[Talk:20.109%28F11%29:_Mod_3_Day_3_TEM | Day 3 Talk page]]. I also have access to the elemental analysis (but not elemental mapping) if anyone wants to request theirs. [[User:AgiStachowiak|AgiStachowiak]] 10:56, 2 December 2011 (EST)
*Primers that are available for sequencing can be found [[Media:ExistingPrimers.pdf| here.]]
*TEM images from T/R are (finally!) on the [[Talk:20.109%28F11%29:_Mod_3_Day_3_TEM | Day 3 Talk page]], with W/F images to come tonight or perhaps tomorrow morning. Enjoy!
*W/F sequencing data is now available. Note that you must log in as "astachow" rather than "nkuldell" (at mit...) and otherwise the M2D6 instructions apply.
*Your TEM images will require special file conversion/exporting before they can be posted here, which it was not possible to do today. Further updates to come tomorrow. <font color = red> Due to the delay, this FNT assignment will be due next week (Tuesday/Wednesday) rather than this week (Thursday/Friday). It will be a bonus FNT assignment worth 4 points rather than required assignment. </font color>
*Agi's usual 2-3 pm office hours are today (in 16-319). All are welcome. W/F section, you can pick up your quizzes there, and must hand in any late lab notebooks by then.
*TEM images will be posted for T/R lab on Wed afternoon. Check here for the link you'll need to access them! And since you're getting these 24 hours later than usual, you can turn in your FNT assignment up to 24 hours late w/o penalty!
*T/R sequencing data is available to examine on GENEWIZ site. Instructions for accessing data are detailed in [[20.109(F11): Mod 2 Day 6 Readouts of DNA, Protein| M2D6]] protocol.
 
*Breaking news: library concentration is 0.4 ug/ul. Sorry this wasn't on M2D3 originally (it is now!)
*T/R M3D1 research ideas available for pick up from box outside 16-325.
*Howard Silver's notes about references are linked to the [[20.109(F11): Fall 2011 schedule| schedule page]] for M2D5
 
*W/F data has been added to the [[Talk:20.109(F11): Mod 2 Day 2 Measuring system performance| M2D2 Talk page]]. I have left space for each team to note any irregularities (A420 out of linear range, realized you didn't mix cells well, &Delta;t was really short, etc.) or other relevant information.
*No lecture today 11.22.11 (have a great T-day)
*Class b-gal data for the bacterial photography system is posted [[Talk:20.109(F11): Mod 2 Day 2 Measuring system performance| here]]
 
*Module 2 journal club sign-up is on the [[20.109%28F11%29:Guidelines_for_oral_presentations | oral presentation guidelines]] page. You may sign up for Day 4 or Day 8 here.
*Mod2 drafts are commented and graded and posted to Stellar (11.22.11). An email with general comments for the whole class will go out later today.  
 
*You can find old announcements [[20.109(F11): old announcements| here]]
*You can find old announcements [[20.109(F11): old announcements| here]]
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<center>Beautiful artwork for homepage from [http://www.chrysosdesigns.com Jennifer Cook-Chrysos]</center>
<center>Beautiful artwork for homepage from [http://www.chrysosdesigns.com Jennifer Cook-Chrysos]</center>

Latest revision as of 10:07, 6 December 2011


20.109(F11): Laboratory Fundamentals of Biological Engineering

Home        People        Schedule Fall 2011        Assignments        Lab Basics        OWW Basics       
DNA Engineering        System Engineering        Biomaterials Engineering              

Fall 2011

Instructors: Angela Belcher, Bevin Engelward, Natalie Kuldell and Agi Stachowiak
Writing Instructor: Linda Sutliff
Oral Presentation Instructor: Atissa Banuazizi
Graduate TAs: Timothy Helbig, Marcus Parrish, Jingjing Sun

Lecture: T/R 11-12 (4-153)
Lab: T/R 1-5 or W/F 1-5 (56-322)
Seminar Room: 16-336

Welcome to 20.109! For many of you this will be the first time in a research lab and for others it will not, but it is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. There is not time enough to show you everything you’ll need to know if you go on to do research, but after taking this class you should feel confident and familiar with some fundamental experimental approaches and lab protocols. You will develop good habits at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with report writing, notebook keeping and publicly presenting your data. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

  • To submit your major assignments, access the Stellar drop-box that's here

Announcements

  • Blogs for Mod3 due Thursday/Friday!!
  • Re-write grades are posted to Stellar Site. Overall the papers were much improved...congrats to you all!
  • Agi's Monday office hour is cancelled. I'm in no shape to be breathing near anyone; I'm happy to discuss research proposal questions over email instead.
  • Final TEM update! W/F images have joined T/R at the Day 3 Talk page. I also have access to the elemental analysis (but not elemental mapping) if anyone wants to request theirs. AgiStachowiak 10:56, 2 December 2011 (EST)
  • TEM images from T/R are (finally!) on the Day 3 Talk page, with W/F images to come tonight or perhaps tomorrow morning. Enjoy!
  • Your TEM images will require special file conversion/exporting before they can be posted here, which it was not possible to do today. Further updates to come tomorrow. Due to the delay, this FNT assignment will be due next week (Tuesday/Wednesday) rather than this week (Thursday/Friday). It will be a bonus FNT assignment worth 4 points rather than required assignment.
  • TEM images will be posted for T/R lab on Wed afternoon. Check here for the link you'll need to access them! And since you're getting these 24 hours later than usual, you can turn in your FNT assignment up to 24 hours late w/o penalty!
  • T/R M3D1 research ideas available for pick up from box outside 16-325.
  • No lecture today 11.22.11 (have a great T-day)
  • Mod2 drafts are commented and graded and posted to Stellar (11.22.11). An email with general comments for the whole class will go out later today.
  • You can find old announcements here
Beautiful artwork for homepage from Jennifer Cook-Chrysos
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