20.109(F11):DNA engineering powerpoint summary

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20.109(F11): Laboratory Fundamentals of Biological Engineering

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Recall that you have been preparing for the powerpoint summary all along. As "For Next Time" assignments, you have:

  • Figure 1: a plasmid map of the clone you have been trying to create. This figures must include all the restriction sites that will be introduced. The slide should also list the sizes you would predict for the fragments generated by an EcoRV/XbaI double digest and a BamHI/XhoI double digest.
  • Figure 2: a properly labeled figure of your pCX-NNX/PCR product agarose gel. This figure should include a legend.
  • Table 1: a table with the results of your ligations and transformations, including a calculation of the transformation efficiency (# colonies/μg plasmid DNA), and a short interpretation of the ligation results.
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