20.109(S07): DNA ligation and bacterial transformation

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20.109: Laboratory Fundamentals of Biological Engineering

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Introduction

Protocols

Part 1: Cloning

  1. ligate
  2. ppt ligations
  3. transform XL1-b. Plate on LB+Kan
  4. label tubes so TA can set up ONs

Part 2: Infecting cells with phage

  1. titer phage in sup
  2. plate infected cells on LB+Kan

DONE!

For next time

Reagents list

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