20.109(S07): Examine candidate clones

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20.109: Laboratory Fundamentals of Biological Engineering

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Introduction

Protocols

Part 1: Cloning

  1. miniprep (save sup, save rest of ONs for Western next time)
  2. digest
  3. agarose gel
  4. send best candidate to sequence

Part 2: Infecting cells with phage

  1. plate sup from all candidates for plaques (during gel run)

DONE!

For next time

Reagents list

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