20.109(S07): Measuring calcium in vivo

Introduction

Microscopy can reveal aspects of cellular structures that other techniques cannot address. Information about a cell's shape, its cellular compartments and the components of those compartments are all routinely collected thanks to laboratory microscopes no different than the one you'll use today. Furthermore, as research to improve microscopy techniques continues, breakthroughs are reported that improve digital image analysis and that increase detection limits imposed by light's diffraction , for example the recent description of PALM microscopy.

Protocols

Microscopy

1. visualize cells vs controls
2. modulate Ca++ with ionophores/chelators

DONE!