20.109(S07): Probe western: Difference between revisions
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==For next time== | ==For next time== | ||
# Prepare for the start of Module 2 by browsing through [[20.109:Module 2]], looking in detail at the introduction for [[20.109(S07): Start-up signal measurement]] | # Prepare for the start of Module 2 by browsing through [[20.109:Module 2]], looking in detail at the introduction for [[20.109(S07): Start-up signal measurement]] | ||
# Read about calmodulin ("CaM") at [[http://www.rcsb.org/pdb/ | # Read about calmodulin ("CaM") at [[http://www.rcsb.org/pdb/ the protein data bank]] (try searching for "molecule of the month calmodulin" for a nice summary). You should also familiarlize yourself with the structure of calmodulin by reading the original description in the 1998 paper by Babu, Bugg, and Cook: [[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WK7-4DN8TPB-3G&_coverDate=11%2F05%2F1988&_alid=500453928&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6899&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=45b82badcf013a46cd97ab08b0114d61]] | ||
# Familiarize yourself with cell culture work by reading [[20.109(S07):Guidelines for working in the tissue culture facility]] | # Familiarize yourself with cell culture work by reading [[20.109(S07):Guidelines for working in the tissue culture facility]] | ||
==Reagents list== | ==Reagents list== |
Revision as of 10:32, 5 December 2006
Introduction
Protocols
Part 1: Cloning
- probe western blot
Part 2: Genome Redesign
- order genome for synthesis
- hand in assignment
DONE!
For next time
- Prepare for the start of Module 2 by browsing through 20.109:Module 2, looking in detail at the introduction for 20.109(S07): Start-up signal measurement
- Read about calmodulin ("CaM") at [the protein data bank] (try searching for "molecule of the month calmodulin" for a nice summary). You should also familiarlize yourself with the structure of calmodulin by reading the original description in the 1998 paper by Babu, Bugg, and Cook: [[1]]
- Familiarize yourself with cell culture work by reading 20.109(S07):Guidelines for working in the tissue culture facility