20.109(S07): Probe western

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20.109: Laboratory Fundamentals of Biological Engineering

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Genome Engineering        Biophysical Signal Measurement        Expression Engineering        Biomaterial Engineering       

Introduction

Protocols

Part 1: Cloning

  1. probe western blot

Part 2: Genome Redesign

  1. order genome for synthesis
  2. hand in assignment

DONE!

For next time

  1. Prepare for the start of Module 2 by browsing through 20.109:Module 2, looking in detail at the introduction for 20.109(S07): Start-up signal measurement
  2. Read about calmodulin ("CaM") at [the protein data bank] (try searching for "molecule of the month calmodulin" for a nice summary). You should also familiarlize yourself with the structure of calmodulin by reading the original description in the 1998 paper by Babu, Bugg, and Cook: [[1]]
  3. Familiarize yourself with cell culture work by reading 20.109(S07):Guidelines for working in the tissue culture facility

Reagents list

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