20.109(S08):Module 2: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: Module 2 Day 1: Start-up protein engineering<br> (Initial reading about CaM-M13? Get calcium titration curve for WT plasmid? Need this day?...) |
No edit summary |
||
Line 1: | Line 1: | ||
{{Template:20.109(S08)}} | |||
<div style="padding: 10px; width: 640px; border: 5px solid #FFCC33;"> | |||
[[20.109(S08):Start-up protein engineering | Module 2 Day 1: Start-up protein engineering]]<br> | [[20.109(S08):Start-up protein engineering | Module 2 Day 1: Start-up protein engineering]]<br> |
Revision as of 14:59, 10 October 2007
Module 2 Day 1: Start-up protein engineering
(Initial reading about CaM-M13? Get calcium titration curve for WT plasmid? Need this day?)
Module 2 Day 2: Design protein modification
(Protein viewer, further reading about CaM-M13 to choose targets, plans primers for SDM)
Module 2 Day 3: Site-directed mutagenesis
(Staff or students do transformation/miniprep/transformation work? Timing issues, may take another lab day...)
Module 2 Day 4: Characterize protein expression
(IPTG induction, SDS-PAGE)
Module 2 Day 5: Protein binding assays
(Calcium titration curve, fluorescence-based)
Module 2 Day 6: Sequence analysis
(Also lipofect wild-type plasmid to mammalian cells?)
(Microscopy assays with mammalian cells? MATLAB modeling?)