20.109(S08):Module 2 Portfolio: Difference between revisions
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==Part 1: Description of IPC mutant design== | ==Part 1: Description of IPC mutant design== | ||
Whether you are writing a grant proposal, seeking venture capital for a new business, or collaborating on almost any project, it’s important to have an awareness of and an ability to describe the key elements of your thought process. Moreover, it is often necessary to address multiple audiences at once. The goal of this writing assignment is to do just that. You should provide enough background (about inverse pericam, mutagenesis, etc.) for a technically adept reader who is ''not'' in the field to understand your protein design goals. You should also provide enough technical detail for someone familiar with calmodulin to know exactly what portion of the protein you are modifying and why. Feel free to talk about design choices that you did not ultimately pursue, to give an idea of the breadth of options before you. However, do not let these become a distraction from the more in-depth discussion of your two particular mutants. Please cite references as appropriate. (You are not required to find references besides the ones given to you in class.) This should be a brief, ~2 page summary, and will be worth | Whether you are writing a grant proposal, seeking venture capital for a new business, or collaborating on almost any project, it’s important to have an awareness of and an ability to describe the key elements of your thought process. Moreover, it is often necessary to address multiple audiences at once. The goal of this writing assignment is to do just that. You should provide enough background (about inverse pericam, mutagenesis, etc.) for a technically adept reader who is ''not'' in the field to understand your protein design goals. You should also provide enough technical detail for someone familiar with calmodulin to know exactly what portion of the protein you are modifying and why. Feel free to talk about design choices that you did not ultimately pursue, to give an idea of the breadth of options before you. However, do not let these become a distraction from the more in-depth discussion of your two particular mutants. Please cite references as appropriate. (You are not required to find references besides the ones given to you in class.) This should be a brief, ~2 page summary, and will be worth 25 % of your portfolio grade. | ||
==Part 2: Summary of Results== | ==Part 2: Summary of Results== | ||
Revision as of 12:58, 11 January 2008
For this module, you will not write a complete lab report. Instead, you will turn in a few independent pieces of written work that provide a summary of and context for your results. The first part of your portfolio is a summary of your design process. It won’t cover precisely the same territory as a traditional Introduction, but should provide a naïve reader with some background necessary for understanding your choices. The second part of your portfolio is essentially a combined Results+Discussion section, but an abbreviated and slightly less formal one, according to the format below.
Part 1: Description of IPC mutant design
Whether you are writing a grant proposal, seeking venture capital for a new business, or collaborating on almost any project, it’s important to have an awareness of and an ability to describe the key elements of your thought process. Moreover, it is often necessary to address multiple audiences at once. The goal of this writing assignment is to do just that. You should provide enough background (about inverse pericam, mutagenesis, etc.) for a technically adept reader who is not in the field to understand your protein design goals. You should also provide enough technical detail for someone familiar with calmodulin to know exactly what portion of the protein you are modifying and why. Feel free to talk about design choices that you did not ultimately pursue, to give an idea of the breadth of options before you. However, do not let these become a distraction from the more in-depth discussion of your two particular mutants. Please cite references as appropriate. (You are not required to find references besides the ones given to you in class.) This should be a brief, ~2 page summary, and will be worth 25 % of your portfolio grade.
Part 2: Summary of Results
Summarize your results in a series of figures/tables with expanded figure captions. As usual, these captions will include: a concise, meaningful title; only as much Methods as is needed to understand the result; clear labeling and definition of all parts. In contrast to a normal figure caption, you may interpret your data in the caption itself (hence ‘expanded’) rather than in a separate Results and Discussion text. Introduce or conclude the list of figures with a short paragraph summarizing your findings, and describe in a couple of sentences what you would try next if you had another month to spend on this project. Your figures may include but are not limited to:
- Schematic indicating your design strategy for mutagenesis
- Table of (or sentence about) colony counts for mutants
- Table of (or sentence about) cell pellet observations
- Figure depicting sequence analysis
- Figure of SDS-PAGE results
- (Table of purified protein concentrations, if available)
- Titration curves for WT and mutant proteins, table of KDs
Part 3:
Written accompaniment to oral presentation? With particular focus on significance/impact of paper? Guided critique?
